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细菌菌子对低压等离子体灭菌的多因素耐药性

Erika Muratov¹, Florian P Rosenbaum², Felix M Fuchs³

¹Radiation Biology Department, Aerospace Microbiology, Institute of Aerospace Medicine, German Aerospace Center (DLR e.V.), Cologne, Germany.

Applied and environmental microbiology

|December 19, 2023

概括

冷低压等离子体 (LPP) 为敏感材料提供了有效的灭菌替代方案. 这项研究调查了Bacillus subtilis子如何抵抗LPP,确定了关键的DNA修复和子生存中的保护蛋白.

科学领域:

微生物学微生物学
等离子体物理学的物理学
生物技术是生物技术.

背景情况:

在医疗,制药和工业领域,不稳定材料的灭菌至关重要.
热量,化学品和辐射等传统方法具有局限性,包括物质损害和环境风险.
冷低压等离子体 (LPP) 是一种具有可调节参数的新兴灭菌技术.

研究的目的:

为了研究 Bacillus subtilis 子对冷低压等离子体 (LPP) 的敏感性.
阐明DNA修复机制和保护蛋白在子抵抗LPP中的作用.
了解背后的子抵抗LPP消毒的机制.

主要方法:

测试了野生型Bacillus subtilis子和突变体缺乏DNA修复 (NHEJ) 或保护性蛋白质 (SASP,catalase) 的LPP易感性.
在不同条件下对LPP治疗后分析了子存活率.
研究了DNA修复,SASP和催化酶对子耐药性的贡献.

主要成果:

治疗LPP显著降低了子的生存能力.
子生存归因于发芽后高效的DNA修复,SASP保护DNA,以及对活性氧物种的催化酶活性.
耐药性机制因LPP的组成和治疗时间而异.

结论:

冷低压等离子体 (LPP) 是一种可行的方法,可以使子形成的细菌失活.
了解子抵抗机制是优化LPP有效消毒的关键.
液压发电提供了一个有希望的,不那么有害的替代品,用于消毒敏感材料.

关键词:

这种细菌是 Bacillus subtilis.清除污染的方法血失活的情况子的抵抗性子的抵抗性

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