选择的cachaça酵母菌株具有与工业发酵过程相关的特征相关的基因组特征
Anna Clara Silva Campos1, Thalita Macedo Araújo1,2, Lauro Moraes3
1Laboratório de Biologia Celular e Molecular, Departamento de Farmácia, Escola de Farmácia, Ouro Preto, Brazil.
Applied and environmental microbiology
|December 19, 2023
概括
研究人员在11个用于生产卡卡萨的Saccharomyces cerevisiae菌株中发现了共享的遗传变异. 这些标记物与巴西精神中改善的发酵,耐压力和风味化合物开发有关.
科学领域:
- 微生物学与发酵科学 微生物学与发酵科学
- 基因组学和分子生物学
- 食品科学与技术 食品科学与技术
背景情况:
- 酵母菌株的选择对于优化巴西烈酒cachaça的质量至关重要.
- 关键特征包括耐受发酵压力因素,挥发性化合物生产和乙醇产量.
- 在cachaça酵母中这些可取的特征的遗传基础尚未完全理解.
研究的目的:
- 为了确定在cachaça发酵中使用的精选的Saccharomyces cerevisiae菌株中常见的特定遗传变异.
- 将这些遗传变异与关键发酵表型联系起来.
- 建立用于酵母菌株选择和潜在遗传改进的分子标记物.
主要方法:
- 11个不同的Saccharomyces cerevisiae菌株的全基因组测序,这些菌株被分离用于cachaça生产.
- 在所有测序的基因组中识别和分析共享的单核酸变体.
- 使用丰富分析和蛋白质-蛋白质相互作用网络对新型和误解变体的功能注释.
主要成果:
- 在所有11个cachaça酵母菌株中共享了20128个单核酸变体.
- 确定了37种新型变体 (包括6种误解) 和4,451种误解变体.
- 具有这些变异的基因与花,耐应力,挥发性化合物生产和乙醇产量有显著的关联.
结论:
- 已经确定了这些cachaça酵母菌株共享的独特遗传特征.
- 这些遗传变异作为潜在的分子标记,用于选择优质的酵母菌株进行发酵.
- 这些发现支持有针对性的基因改进策略,以提高cachaça的质量和生产.
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