假定自身六倍体Actinidia deliciosa的染色体级基因组提供了对多倍体化和进化的洞察力
Yongbo Liu1, Yi Zhou1, Feng Cheng2
1State Key Laboratory of Environmental Criteria and Risk Assessment, State Environmental Protection Key Laboratory of Regional Eco-process and Function Assessment, Chinese Research Academy of Environmental Sciences, 8 Dayangfang, Beijing, 100012, China.
The Plant journal : for cell and molecular biology
|December 19, 2023
概括
六倍体Actinidia deliciosa基因组被测序,揭示了它与二倍体Actinidia chinensis的密切关系. 尽管有证据表明自身六合体化,但染色体重组表明这种重要的水果作物中复杂的基因组进化.
科学领域:
- 植物基因组学 植物基因组学
- 多类动物的进化过程.
- 作物遗传学作物遗传学
背景情况:
- 包括Actinidia chinensis (二倍体) 和Actinidia deliciosa (六倍体) 在内的Actinidia物种是重要的水果作物.
- 假设Actinidia deliciosa是一种自制六合体,源自像A. chinensis. chinensis这样的二合体祖先.
- 了解A. deliciosa的基因组结构对于其改进和进化研究至关重要.
研究的目的:
- 为六倍体Actinidia deliciosa提供染色体解析的基因组组.
- 为了研究A. deliciosa及其假定双体祖先之间的进化关系.
- 探索A. deliciosa.适应和多样化的基因组基础.
主要方法:
- 使用基于CCS的组装方法,固定在高分辨率的链接地图上.
- 进行了合成的基因组内和基因组间比较.
- 分析了基因表达模式和人口扩张历史.
主要成果:
- 为A. deliciosa生成了3.91Gb的基因组组,包括174个伪染色体,分为29个同源组.
- 在六倍体A. deliciosa基因组和二倍体A. chinensis之间发现了很高的相似性 (95.5%的同源基因对>90%的相似性).
- 鉴定出染色体重排,表明在自身倍积分后发生了结构变化.
- 观察到多样化的基因表达和证据表明,在多化后人口迅速扩张.
结论:
- A. deliciosa的基因组与A. chinensis显著相似,但包含了染色体重排的证据.
- 在A. deliciosa.中,染色体重新排列可能发生在A. deliciosa.中自制化后.
- 基因表达多样化和人口扩张促进了A. deliciosa.的适应和利基区分.
- 以等位基因定义的六倍体基因组为作物改进和多倍体进化研究提供了宝贵的资源.
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