ангиотензин-(1-7) 改善了骨肌肉的再生
Mayalen Valero-Breton1, Franco Tacchi2, Johanna Abrigo3
1Laboratory of Muscle Pathology, Fragility and Aging, Faculty of Life Sciences, Universidad Andres Bello, Santiago, Chile; Millennium Institute on Immunology and Immunotherapy, Faculty of Life Sciences. Universidad Andres Bello, Santiago. m.valerobreton@uandresbello.edu.
European journal of translational myology
|December 19, 2023
概括
ангиотензин (1-7) [Ang-(1-7) ]通过增强肌管分化和增加新的肌肉纤维大小来促进骨肌肉再生. 这种可以通过改善受伤后的肌肉修复来治疗肌肉疾病的潜力.
科学领域:
- 肌肉生理学和肌肉再生
- 内分泌学和信号传递.
- 细胞生物学 细胞生物学
背景情况:
- 骨肌肉的再生依赖于卫星细胞,但在肌肉发育不良症中受损.
- ангиотензинII (Ang-II) 与病理状态有关,而 ангиотензин (1-7) [Ang-(1-7) ] 通过Mas受体具有抵消作用.
- -1-7在骨肌肉再生中的作用尚不清楚.
研究的目的:
- 调查Ang-(1-7) 在骨肌肉再生中的特定作用.
- 为了在体外确定Ang-(1-7) 对C2C12髓母细胞分化的影响.
- 在体内评估Ang-(1-7) 对肌肉损伤后修复的影响.
主要方法:
- 分化了C2C12神经细胞与或没有Ang-(1-7) 以评估肌管直径和关键肌肉蛋白水平 (肌原蛋白,肌重链[MHC]).
- 在C57BL/6小鼠中使用化诱导骨肌损伤.
- Ang-(1-7) 给受伤的小鼠,随后进行组织学分析和胚胎肌肉蛋白 (eMHC) 和肌肉蛋白蛋白水平的评估.
主要成果:
- 在体外,Ang-(1-7) 治疗导致C2C12神经管直径增加以及肌原蛋白和MHC的水平升高.
- 在体内,Ang-(1-7) 管理增强了肌肉再生,由更多的新肌肉纤维和更大的直径证明,包括eMHC阳性纤维.
- 蛋白质分析证实Ang-(1-7) 治疗受伤肌肉中的eMHC和肌原蛋白水平升高.
结论:
- Ang-(1-7) 在体外促进C2C12细胞分化.
- Ang-(1-7) 剂量显著改善了小鼠受伤后的骨肌肉再生.
- 这些发现表明Ang-(1-7) 对肌肉相关疾病具有治疗潜力.
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