相关实验视频
Updated: Jul 8, 2025
00:07
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
7.3K
通过调节miR-206/HMGA2表达,LncRNAHAGLROS有助于皮肤状甲状腺癌的进展
Zhaohui Zeng1, Shengtao Tang2, Liping Chen1
1Department of Nuclear Medicine, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410001, Hunan, China.
Aging
|December 19, 2023
概括
长非编码RNAHAGLROS通过与miR-206相互作用并上调HMGA2.2,促进乳头甲状腺癌 (PTC) 的生长. 这种分子途径为内分泌系统疾病提供了潜在的治疗点.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 乳头甲状腺癌 (PTC) 对内分泌系统构成重大威胁.
- 目前用于PTC的治疗方法的疗效有限,需要新的治疗策略.
- 研究分子相互作用为新的治疗途径提供了潜力.
研究的目的:
- 阐明PTC进展背后的分子机制.
- 通过研究HAGLROS/miR-206/HMGA2相互作用来确定潜在的治疗点.
- 在分子层面探索新的治疗策略.
主要方法:
- 多omics分析以确定差异表达的RNA (lncRNA,miRNA,mRNA).
- 定量逆转录聚合酶链反应 (qRT-PCR) 用于RNA表达的验证.
- 双露西法酶记者测定以确认RNA结合关系.
- 细胞测试 (殖民地形成,流细胞计,Transwell) 来评估功能影响.
- 使用小鼠模型进行体内研究以评估治疗效果.
主要成果:
- 在PTC组织和细胞中,HAGLROS和HMGA2的上调,而miR-206的下调.
- 哈格洛斯和HMGA2促进PTC细胞的增殖和迁移,同时抑制细胞亡.
- miR-206表现出相反的效果,促进细胞灭绝并抑制增殖/迁移.
- 在HAGLROS/HMGA2和miR-206表达之间观察到负相关性.
- 沉默HAGLROS降低了HMGA2的表达,并在体内抑制了瘤的生长.
结论:
- 哈格洛斯通过竞争性结合miR-206促进PTC生长,从而增加HMGA2表达.
- HAGLROS/miR-206/HMGA2轴代表了对乳头甲状腺癌的有前途的治疗标.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
02:55The Nucleolus
8.9K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
8.9K