Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.1K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
11.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Human-scATAC-Corpus: a comprehensive database of scATAC-seq data.

Nucleic acids research·2025
Same author

Partitioning and aggregating cross-tissue and tissue-specific genetic effects to identify gene-trait associations.

Nature communications·2024
Same author

Global age-structured spatial modeling for emerging infectious diseases like COVID-19.

PNAS nexus·2023
Same author

Quantifying portable genetic effects and improving cross-ancestry genetic prediction with GWAS summary statistics.

Nature communications·2023
Same author

CITEdb: a manually curated database of cell-cell interactions in human.

Bioinformatics (Oxford, England)·2022
Same author

Differential analysis of RNA structure probing experiments at nucleotide resolution: uncovering regulatory functions of RNA structure.

Nature communications·2022
Same journal

STED: flexible cross-modal topic modeling infers cell-type-specific regulatory landscapes from bulk epigenomics.

Briefings in bioinformatics·2026
Same journal

A knowledge-guided deep learning framework for quantitative nucleic acid testing.

Briefings in bioinformatics·2026
Same journal

Optimal transport for label transfer in single-cell multi-omics integration.

Briefings in bioinformatics·2026
Same journal

Continuous multi-omics pathway enrichment analysis resolves hidden functional heterogeneity.

Briefings in bioinformatics·2026
Same journal

Evaluating completeness, coherence, and consistency of genome-scale function annotations.

Briefings in bioinformatics·2026
Same journal

Transformers for single-cell RNA sequencing: a survey.

Briefings in bioinformatics·2026
查看所有相关文章

相关实验视频

Updated: Jul 8, 2025

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
08:08

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

Published on: April 2, 2018

11.3K

咖啡:对单细胞染色质可访问性数据的基于相关性特征选择.

Keyi Li1, Xiaoyang Chen1, Shuang Song2

  • 1Ministry of Education Key Laboratory of Bioinformatics, Bioinformatics Division at the Beijing National Research Center for Information Science and Technology, Center for Synthetic and Systems Biology, Department of Automation, Tsinghua University, Beijing 100084, China.

Briefings in bioinformatics
|December 19, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了Cofea,这是一种分析单细胞染色体可访问性测序 (scCAS) 数据的新方法. 咖啡有效捕捉细胞异质性,并有助于从表观基因组数据中发现功能生物学见解.

关键词:
染色质可访问性 染色质可访问性数据预处理数据预处理.显基因组是一个表观基因组.功能选择 功能选择一个单细胞的单细胞.

更多相关视频

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

2.7K
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

18.1K

相关实验视频

Last Updated: Jul 8, 2025

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
08:08

Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

Published on: April 2, 2018

11.3K
Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

2.7K
Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

18.1K

科学领域:

  • 基因组学就是基因组学.
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 计算生物学 计算生物学

背景情况:

  • 单细胞染色体可访问性测序 (scCAS) 提供了关于细胞表观基因组异质性的见解.
  • 在scCAS数据中识别生物相关特征仍然是一个挑战.

研究的目的:

  • 引入Cofea,一种用于分析scCAS数据的新计算方法.
  • 为了解决从scCAS数据集中识别功能特征的差距.

主要方法:

  • 开发并验证了Cofea的方法.
  • 在5个模拟和54个现实世界scCAS数据集上测试了Coffea.
  • 应用 Cofea 来识别细胞类型特定的峰值,候选增强剂,并进行途径和遗传性分析.

主要成果:

  • 与现有方法相比,咖啡在捕捉细胞异质性方面表现优越.
  • 该方法成功地促进了下游分析,包括特征识别和丰富.
  • 实验证实了Coféa在各种数据集中的有效性.

结论:

  • 咖啡是一种强大的工具,可以在单细胞分辨率下剖析表观基因异质性.
  • 该方法增强了从scCAS数据中发现功能生物过程的发现.
  • 咖啡在推进表观基因组研究方面有很大的潜力.