在酸和CO2依赖的口腔调节过程中,明显的护卫细胞特定的染色质可访问性的重塑
Charles A Seller1, Julian I Schroeder1
1School of Biological Sciences, Cell and Developmental Biology Department, University of California San Diego, La Jolla, CA 92093-0116.
概括
酸 (ABA) 在植物护卫细胞中引发显著的染色质重塑,影响抗压力基因表达. 二氧化碳 (CO2) 对这些染色质动态的影响很小.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 防护细胞通过控制口腔孔来调节植物气体交换.
- 染色体结构影响转录因子结合和基因调节.
- 在口腔运动和ABA反应期间,护卫细胞中大规模染色质重塑的作用以前是未知的.
研究的目的:
- 为了研究酸 (ABA) 和二氧化碳 (CO2) 是否调节*Arabidopsis thaliana*的口腔运动期间的护卫细胞染色素.
- 为了确定细胞类型特定的染色质可访问性模式和护卫细胞中的 cis 调节序列.
- 阐明了ABA和CO2诱导的染色质改造背后的分子机制.
主要方法:
- 从Arabidopsis thaliana*中分离了护卫细胞核.
- 对染色质可访问性的细胞类型特定分析.
- 分析DNA基因,以确定转录因子结合部位.
- 研究特定转录因子 (ABF,bHLH) 在ABA介导的染色质变化中的作用.
主要成果:
- 在护卫细胞,根细胞和中细胞中,ABA诱导了广泛和动态的染色质重塑,具有特定细胞类型的模式.
- 不同的转录因子结合部位富含ABA诱导和ABA抑制的染色质.
- 沉酸反应元件 (ABRE) 结合因子 (ABF) bZIP型TFs对于ABA触发的护细胞和根中的染色体开放至关重要.
- 抑制bHLH型TFs的一个基团与控制ABA抑制的染色质有关.
- ABA和CO2诱导不同的染色质重塑程序;高的CO2的影响最小.
结论:
- 护卫细胞中ABA诱导的染色质重塑是一个动态的过程,涉及特定的转录因子.
- 不同的环境信号,如ABA和CO2,会触发不同的染色质重塑路径.
- 由ABA介导的染色质变化可能会使植物基因组产生增强的非生物应激抵抗力.
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