玉米茎强度的遗传基础通过链接和关联映射解码
Binghao Zhao1, Kun Li1,2, Min Wang1
1State Key Laboratory of Plant Environmental Resilience and National Maize Improvement Center of China, China Agricultural University, Beijing, 100193, China.
The Plant journal : for cell and molecular biology
|December 19, 2023
概括
的住宿严重限制了玉米的生产. 这项研究确定了影响皮肤透电阻 (RPR) 的定量特征基因 (QTL) 和候选基因,为开发耐寄存的玉米品种提供了洞察力.
科学领域:
- 植物遗传学 植物遗传学
- 农业科学 农业科学
- 玉米育种 玉米育种 玉米育种
背景情况:
- 子寄宿是全球玉米生产的主要限制,但其遗传基础仍未得到充分探索.
- 皮层透计阻力 (RPR) 是评估玉米中茎的阻力的一个关键指标.
研究的目的:
- 通过链接和关联映射,研究玉米中RPR的遗传结构.
- 识别与RPR相关的定量特征基因 (QTL) 和候选基因,以促进耐寄存玉米品种的发展.
主要方法:
- 在两大玉米种群中测量了RPR:一个多亲种群 (1948年RILs) 和一个协会种群 (508个杂交系).
- 使用链接和关联映射方法来检测RPR的单个和表皮质QTL.
- 进行了全基因组关联研究 (GWAS),以确定影响RPR的候选基因.
主要成果:
- 在这两个人群中分别发现了53个和29个单个QTL,以及50个和19个RPR的表皮相互作用.
- 单添加性QTL解释了显著更多的表型变异 (高达~60%) 比表皮性QTL (高达~5%).
- 确定了137个候选基因,其中66个经过交叉验证,23个在高RPR线和低RPR线中显示差异性表达.
结论:
- RPR的遗传基础是复杂的,涉及附加性和表观性相互作用.
- 已识别的QTL和候选基因为增强茎抗性的育种玉米提供了宝贵的遗传资源.
- 针对已识别的基因提供了一个分子育种策略,以减轻由于茎存放而导致的产量损失.
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