相关实验视频

Updated: Jul 8, 2025

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification

Published on: November 20, 2017

BFGF通过抑制内分泌网膜压力介导的亡作用来减弱大动脉膜间歇细胞化

Gao Yuan¹, Li Ning², Xue Qing¹

¹Department of Cardiovascular Surgery, The First Affiliated Hospital of Naval Medical University, Shanghai, 200433, China.

Experimental cell research

|December 19, 2023

概括

基本纤维细胞生长因子 (BFGF) 通过抑制膜间歇细胞中的骨质分化和亡来降低状动脉病 (CAVD). 这种保护作用涉及PI3K/Akt和ERK1/2通路.

科学领域:

心血管生物学心血管生物学
细胞机制细胞机制
翻译医学是一种翻译医学.

背景情况:

动脉疾病 (CAVD) 是一种进展性疾病,治疗选择有限.
基本纤维细胞生长因子 (BFGF) 在CAVD病变发生中的作用及其潜在机制仍然不清楚.
在之前的研究中,BFGF已经显示出潜在的心血管保护作用.

研究的目的:

研究BFGF在缓解CAVD中的治疗潜力.
阐明BFGF影响膜间歇细胞 (VIC) 骨质分化和亡的分子机制.
评估BFGF在CAVD的临床前小鼠模型中的疗效.

主要方法:

猪VICs被隔离并用骨质原生诱导介质 (OIM) 和BFGF进行治疗.
蛋白质表达被分析使用西方污点;通过流细胞计评估了细胞亡.
建立了一种大鼠CAVD模型,并用外源BFGF进行治疗,评估包括心声回声和阿利沙林红色染色.

主要成果:

在CAVD患者的大动脉门和血清中,BFGF水平升高.
外源性BFGF的使用在体内减轻了CAVD的进展.
BFGF抑制了骨质分化,内细胞网膜应激 (ERS) 和VIC中的亡,PI3K/Akt和ERK1/2通路抑制剂消除了这些效应.

结论:

BFGF可以缓解VIC化和CAVD进展.
通过抑制ERS诱导的亡,BFGF的保护作用受到介导.
激活PI3K/Akt和ERK1/2信号通路在BFGF的治疗作用中起着至关重要的作用,这表明BFGF是潜在的CAVD治疗方法.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.基本的纤维细胞生长因子动脉的疾病动脉疾病细胞内膜网膜应激压力是什么门间歇细胞的间歇细胞

更多相关视频

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro

Published on: May 10, 2021

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Isolation of Human Primary Valve Cells for In vitro Disease Modeling

Published on: April 16, 2021

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...