相关实验视频

Updated: Jul 8, 2025

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Determination of the Mating Efficiency of Haploids in Saccharomyces cerevisiae

Published on: December 2, 2022

单向配对类型的交换是由保存的MAT1位点架构支的

P Markus Wilken¹, Frances A Lane¹, Emma T Steenkamp¹

¹Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Pretoria 0028, South Africa.

Fungal genetics and biology : FG & B

|December 19, 2023

概括

在真菌中单向交配类型的切换涉及保存的MAT1位点结构. 这项研究揭示了Ceratocystidaceae中MAT1-2基因安排如何促进这种独特的繁殖策略.

科学领域:

菌类学菌类学是指菌类学.
进化生物学进化生物学
遗传学是一个遗传学.

背景情况:

单向交配型切换是一种在一些丝状虫中发现的繁殖策略.
这一过程产生了自我肥沃或自我无菌的真菌分离物.
交配类型的特异性差异与MAT1位点架构有关.

研究的目的:

为了研究Ceratocystidaceae真菌中的MAT1位点结构.
为了确定MAT1位点安排是否支持这种真菌家族的单向交配类型切换.
了解这种生殖战略的演变.

主要方法:

使用了遗传和基因组方法.
在Ceratocystis,Endoconidiophora和Davidsoniella的关键物种中注释了MAT1位点.
在自我肥沃和自我无菌的隔离物中对MAT1位点结构进行比较分析.

主要成果:

在自我生育的分离体中,MAT1-2基因在MAT1位点内始终中断MAT1-1-1基因.
围绕MAT1-2基因的直接重复表明了重组介导的删除机制.
这种MAT1-1-1基因表达的破坏和随后的恢复与交配型切换相关.

结论:

在Ceratocystidaceae中支持一种与单向交配类型切换相关的保存的MAT1位置结构.
这些发现为推动这种生殖战略的进化机制提供了洞察力.
这项研究增强了对真菌的交配类型决定和进化的理解.

关键词:

在Ceratocystidaceae家族中.切拉托西斯蒂斯 (Ceratocystis) 是一种状囊.戴维斯·索尼埃拉 (Davidsoniella) 是一个美国人.它们中的一个是Endoconidiophora.性繁殖性繁殖的性繁殖.

更多相关视频

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline

Published on: September 13, 2022

相关概念视频

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

The Ratio of X Chromosome to Autosomes

The Ratio of X Chromosome to Autosomes

In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...

Yeast Signaling

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...