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在种子植物中识别miRNA858长环前体

Wen-Qiu Wang^1,2, Xiao-Fen Liu¹, Yong-Jing Zhu¹

¹College of Agriculture and Biotechnology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.

|December 19, 2023

概括

微RNAs (miRNAs) 是植物中的关键调节者. 这项研究确定了果和其他种子植物中miR858的新型长环前体 (MIR858L),影响植物代谢.

科学领域:

植物分子生物学植物分子生物学
转录后的监管转录后的监管
植物的二次新陈代谢

背景情况:

微RNAs (miRNAs) 对植物发育和应激反应至关重要.
MiR858调节了参与二次新陈代谢的MYB转录因子.
之前对miR858前体的研究仅限于短发针,阻碍了跨物种研究.

研究的目的:

系统地研究miR858前体及其在各种植物物种中的功能.
为了识别和表征miR858.8的新型,长环头前体.
阐明MIR858L-MYB模块在植物二次新陈代谢中的作用.

主要方法:

使用宽松的针头识别标准重新评估miR858的转录.
生物信息分析用于在果和其他种子植物中识别长环发针前体 (MIR858L).
在阿拉比多普西斯和基维果中定量氨酸和黄醇水平,以评估MIR858L的功能.

主要成果:

在果 (1,1002,100 nt) 中发现miR858的长环发针前体 (MIR858L).
在种子植物中识别MIR858L同类,长度可变 (3505,500 nt).
证明:MIR858L显著影响了阿拉比多普西斯和桃中的普朗索亚尼丁和黄醇积累.

结论:

在种子植物中,MIR858L前体类代表了广泛的监管元素.
MIR858L-MYB调节模块可能在植物二次新陈代谢的演变中发挥了重要作用.
这一发现扩大了我们对miRNA前体多样性和植物功能的理解.

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...