患者衍生的肺癌"三明治文化"与保存的瘤微环境
Hailong Wang1, Thorsten Walles1, Cornelia Wiese-Rischke1
1University Clinic for Cardiac and Thoracic Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, Germany.
Tissue engineering. Part C, Methods
|December 20, 2023
概括
研究人员使用患者样本开发了一种新的3D肺癌模型,以更好地模拟瘤微环境 (TME),以改善体外药物测试和个性化医学研究.
科学领域:
- 生物医学工程 生物医学工程
- 在瘤学瘤学.
- 3D 细胞培养 3D 细胞培养
背景情况:
- 目前的3D肺癌模型缺乏准确的瘤微环境 (TME) 表示,限制了瘤细胞与TME相互作用的研究.
- 现有的模型包括球形体,器官类型和生物打印系统,但未能完全回顾复杂的TME.
- 来自患者的样本对于开发更相关的体外模型至关重要.
研究的目的:
- 建立一个新的3D体外肺癌模型,使用来自患者的样本.
- 模拟瘤微环境 (TME) 和瘤细胞-TME相互作用.
- 为了实现先进的体外药物测试和个性化医疗方法.
主要方法:
- 从患者衍生的肺瘤样本建立了2D和3D共同培养模型.
- 使用静态和动态培养条件长达28天.
- 使用H&E染色,M30 ELISA和免疫光检测肺癌标记物 (TTF-1,p40/p63),CAF标记物 (α-SMA,MCT4) 和纤维素 (FN) 的特征模型.
主要成果:
- 与2D模型相比,3D模型的成功率更高.
- 静态3D模型显示,从21日到28日,细胞密度增加和细胞亡减少.
- 动态3D模型表现出更高的细胞密度,成功地在复杂的结构中识别了肺癌细胞,CAF和纤维菌素.
结论:
- 一个新的3D体外肺癌模型成功建立,模拟了TME28天.
- 该模型表现出结构复杂性,并允许研究肺癌细胞,CAF和纤维菌素.
- 这种先进的模型有望改善肺癌研究中的体外药物测试和个性化医学.
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