修改的轨道:在单分子测序中可视化修改基的可视化.
Daofeng Li1, Xiaoyu Zhuo1, Jessica K Harrison1
1Department of Genetics, The Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
Cell genomics
|December 20, 2023
概括
新的可视化工具使研究人员能够从长时间读取的测序中探索表观基因组数据. 华盛顿大学表观基因组浏览器中的修改过的轨迹类型显示了个别读取的DNA基的化学修改.
科学领域:
- 基因组学和表观遗传学
- 生物信息学和计算生物学
背景情况:
- 长读数测序技术已经显著进步,提高了读数长度和检测DNA基基修改的准确性.
- 研究表观基因组对于理解基因调节至关重要,但在基因组浏览器中可视化修改基因仍然是一个挑战.
研究的目的:
- 解决从长时间读取的测序数据中可视化修改基的局限性.
- 引入一种新的轨道类型,以加强对表观基因组信息的探索.
主要方法:
- 在WashU Epigenome Browser中开发和实施"modbed"轨道类型.
- 修改过的轨道可视化了在各种分辨率的单读和聚合分子水平上的修改细节.
- 支持本地文件上传和可访问的URL,以实现无数据集成.
主要成果:
- 经过修改的轨道类型成功地使从长时间读取的测序数据中对正规核酸基的化学修饰进行可视化.
- 用户现在可以在单个测序读取和跨多个分子中探索详细的修改模式.
- 华盛顿大学表观基因组浏览器为动态和高分辨率的表观基因组数据可视化提供了一个平台.
结论:
- 修改后的轨道类型显著提高了通过长读序列生成的表观基因组数据的可视化和分析能力.
- 这一进步促进了对表观基因组的更全面的探索,为生物发现开辟了新的途径.
- 华盛顿大学表观基因组浏览器为研究DNA修饰的研究人员提供了一个免费和可访问的工具.
更多相关视频
相关概念视频
01:05Maxam-Gilbert Sequencing
11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
Challenges of the Maxam-Gilbert Method
The...
11.2K
03:00Next-generation Sequencing
88.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.9K
03:21RNA-seq
10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K