通过S-化介导的DJ-1与PTEN的合会诱导PI3K/AKT/mTOR路径依赖的 keloid 形成
Dongming Lv1, Zhongye Xu1, Pu Cheng2
1Department of Burn and Plastic Surgery, the First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou 510080, China.
Burns & trauma
|December 20, 2023
概括
这项研究表明,S-化DJ-1 (SNO-DJ-1) 通过激活PI3K/AKT/mTOR通路来促进 keloid 形成. 准SNO-DJ-1为 keloid 治疗提供了一个潜在的新疗法策略.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 皮肤病学 皮肤病学
背景情况:
- 体代表异常的伤口愈合与过度纤维化和复发.
- 过度活跃的炎症,遗传因素和紧张因素都与 keloid 病原发生有关.
- 目前尚不清楚S-化 (SNO) 在 keloid 形成中的作用.
研究的目的:
- 为了研究蛋白质S-化和 keloid 形成之间的关联.
- 阐明DJ-1蛋白及其S-化在 keloid 病原发生中的作用.
主要方法:
- 纤维细胞从正常和 keloid 组织中分离出来.
- 对细胞增殖,细胞亡,迁移和入侵进行检测.
- 体ID近距离标记,质谱学,生物开关测定和转化化反应来检测蛋白质SNO.
主要成果:
- DJ-1 增强了 keloid 纤维细胞的增殖,迁移和入侵.
- 在依赖Cys106 SNO的 keloids 中发现了高的S-化DJ-1 (SNO-DJ-1) 水平.
- 通过转化PTEN,SNO-DJ-1促进胆固醇的形成,抑制其活性并激活PI3K/AKT/mTOR通路.
结论:
- SNO-DJ-1/SNO-PTEN/PI3K/AKT/mTOR轴对于化物形成至关重要.
- PTEN的DJ-1转尼化是一种促进化体发育的新型机制.
- 化S-化DJ-1为化体治疗提供了一个潜在的治疗标.
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