通过调节碳源供应和活体代谢途径来调节糖化修饰的纤维素的有效和经济利用
Simin Liu1,2, Jiamei Liu1,2, Linguo Zhao1,2
1Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Journal of agricultural and food chemistry
|December 20, 2023
概括
这项研究通过优化纤维素酸化路径来提高糖化效率. 改进的碳源调节和基因淘汰策略显著提高了蜂菌的转化和维特辛的产生.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 酶合成酶的合成
背景情况:
- 糖化是关键的生物修饰,但使用纤维素酸化的体内方法面临较低的转化率.
- 增强纤维素转化是提高糖化效率和产生像维捷克辛这样的有价值化合物的关键.
研究的目的:
- 通过纤维素酸酸化路径提高糖化酶的效率.
- 通过优化蜂菌转化率来增强维特辛的生产.
主要方法:
- 通过调整葡萄糖与蜂糖的比例来调节碳源供应.
- 被淘汰的基因 (pgm,agp,ushA) 参与了UDP-葡萄糖降解.
- 优化转化条件,以增强糖基化.
主要成果:
- 与对照组相比,达到2228 mg/L的最大维捷辛产量,比对照组增加了312%.
- 达到88%的蜂菌转化率,是迄今为止报告的最高水平.
- 证明了葡萄糖添加和基因淘汰对维特辛产量的协同效应.
结论:
- 优化碳源调节和向基因淘汰有效地增强了纤维素菌的转化为糖化.
- 这项研究提出了一种具有成本效益和效率的方法,用于改进甘化过程.
- 开发的战略大大提高了维特辛的生产,提供了一个可扩展的生物技术解决方案.
相关概念视频
What is Glycolysis?
165.0K
Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...
165.0K
Oligosaccharide Assembly
2.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.9K


