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免疫复合体介导的质核突炎的狗中的微RNA-126

Ariana D Cherry¹, Candice P Chu¹, Rachel E Cianciolo²

¹Department of Veterinary Pathobiology, School of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, Texas, USA.

Journal of veterinary internal medicine

|December 20, 2023

概括

尿液miR-126可以帮助识别免疫复合物介导的球炎的狗,而miR-21和miR-182可能表明慢性病的狗的疾病严重程度.

科学领域:

兽医医学兽医医学兽医医学
发现生物标志物的发现.
分子诊断学分子诊断学

背景情况:

患有慢性脏病的蛋白质性狗经常患有粉样性粉症,血球硬化症或免疫复杂介导的血球炎.
对于准确的诊断和预后,需要一种非侵入性,特定于疾病的生物标志物.

研究的目的:

调查生物流体中微RNA (miRNA) 表达模式与狗球细胞疾病的进展/分类之间的相关性.
为了确定潜在的非侵入性生物标志物,以区分狗的球细胞疾病.

主要方法:

备份的血清和尿液样本的回顾性分析,来自生物检验证实了淋巴细胞疾病的狗和健康的对照.
血清和尿路miRNAs的分离和RNA测序.
使用定量逆转录PCR对特定的尿路miRNAs (miR-126,miR-21,miR-182,miR-486) 的量化.

主要成果:

尿中的miR-21和miR-182在亚体狗中显著高,与管间纤维化相关.
尿中的miR-126表达在免疫复合物介导的质炎的狗中明显升高,与质硬化症,粉样化症和健康对照相比.
在疾病阶段之间没有观察到血清miRNA表达的显著差异.

结论:

尿 miR-126 显示出作为生物标志物的潜力,以识别患有免疫复合物介导的淋巴结膜炎的狗,这些狗可能从免疫抑制疗法中受益.
尿中的miR-21和miR-182可以作为狗疾病严重程度和纤维化指标.
这些发现突出了尿路miRNAs作为兽医脏病学中的非侵入性诊断工具的潜力.

关键词:

慢性脏疾病慢性脏疾病在 miR-12626 系统中,这就是miR-182的原因.这就是为什么miR-21 尿液尿液尿液

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...