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可激活的有机探头用于生物分子的现场成像

Ke Li¹, Minghui Yang¹

¹College of Chemistry & Chemical Engineering, Central South University, Changsha, 410083, Hunan Province, China.

Chemistry, an Asian journal

|December 20, 2023

概括

可激活的有机探头能够在现场对活细胞内的生物分子进行高分辨率的成像. 本综述总结了探头设计策略和生物应用,以推进生物医学中的细胞事件解释.

科学领域:

生物化学和分子生物学
细胞生物学细胞生物学
生物医学成像技术生物医学成像技术

背景情况:

生物分子对于细胞过程至关重要,理解它们的动态需要高分辨率的现场成像.
使用可激活的有机探针进行光成像,可提供非侵入性的细胞内化和易于修改以实现目标可视化.
目前在延长成像的细胞内保留光信号方面存在局限性.

研究的目的:

审查可激活的有机探针的一般设计策略,这些探针可以维持细胞内部的光信号.
为了突出这些探头在in situ生物成像中的生物应用.
鼓励开发新型分子工具,用于更广泛的现场成像应用.

主要方法:

对可激活有机探针的现有设计策略的文献审查.
对用于增强细胞保留和光的探针修改的分析.
在生物分子的现场生物成像中报告的生物应用的汇编.

主要成果:

总结了可激活有机探针的一般设计原则,重点关注细胞内信号保留.
介绍了这些探针在活细胞成像中的各种生物应用.
该审查确定了能够有效地进行现场生物成像的关键特征.

结论:

可激活的有机探头是生物分子现场生物成像的强大工具.
战略性探针设计对于成功的细胞内光成像是必不可少的.
这些探头的进一步开发将扩大它们在生物医学研究中的实用性.

关键词:

可激活的有机探头.生物分子生物分子.在现场生物成像.

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