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基于加多的对比剂和自由的加多抑制了骨细胞系的分化和活性
Franziska Strunz1, Christoph Stähli, Johannes T Heverhagen
1From the Bone and Joint Program, Department for BioMedical Research, University of Bern, Bern, Switzerland (F.S., W.H., R.J.E.); Graduate School for Cellular and Biomedical Sciences, University of Bern, Switzerland (F.S.); RMS-Foundation, Bettlach, Switzerland (C.S.); Department of Diagnostic, Interventional, and Pediatric Radiology, University Hospital, Inselspital, University of Bern, Bern, Switzerland (J.T.H., R.J.E.); and Clinic for Cranio-Maxillofacial Surgery, Department for BioMedical Research, University of Bern, Bern, Switzerland (W.H.).
加多 (Gd) 和基于加多的对比剂 (GBCA) 在体外会损害骨细胞的分化和功能. Gd在骨中的保留可能会破坏骨的循环和新陈代谢.
科学领域:
- 生物医学科学 生物医学科学
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 在MRI中使用的基于加多 (Gd) 的对比剂 (GBCA) 在包括骨在内的组织中被保留.
- Gd和GBCA对骨细胞功能和新陈代谢的潜在影响尚未完全理解.
研究的目的:
- 在体外研究Gd和GBCA对骨细胞系的增殖,生存,分化和功能的影响.
- 评估不同类型的GBCA (线性和宏循环) 对骨细胞的影响.
主要方法:
- 培养了原发性小鼠骨质母细胞 (OB) 和骨质母细胞原生细胞 (OPC).
- 细胞暴露于Gd3+的不同度和三种类型的GBCA:Gd-DTPA-BMA,Gd-DTPA和Gd-DOTA.
- 分析了细胞活力,分化和骨质细胞吸收活性.
主要成果:
- Gd3+减少了依赖剂量的骨质母细胞分化,但没有影响生存能力.
- GBCA对骨质细胞活力的影响较小,但显著降低了分化,Gd-DTPA-BMA具有最明显的影响.
- Gd3+降低了骨质细胞原体的分化,而GBCA增加了生存能力,但降低了分化.
- 当Gd被纳入骨基质时,骨质细胞的吸收活性降低.
结论:
- 加多和GBCA在体外抑制骨细胞系的分化和活性.
- 骨组织中Gd的保留可能会损害细胞水平的骨循环调节.
- 由于Gd的积累,可能导致骨代谢的病理变化.
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