在哺乳动物进化过程中,MAIT细胞的保存转录程序
Hélène Bugaut1, Yara El Morr1, Martin Mestdagh1
1Institut Curie, Paris Sciences et Lettres University, Institut National de La Santé et de La Recherche Médicale U932, Immunity and Cancer , Paris, France.
The Journal of experimental medicine
|December 20, 2023
概括
粘膜关联不变T细胞 (MAIT) 在不同物种中表现出保存的成熟,标记为ZBTB16. 祖先的MAIT细胞共享一种类型-1/17程序,而小鼠肠道的MAIT细胞获得了独特的细胞毒性和组织修复功能.
科学领域:
- 免疫学 免疫学 免疫学
- 进化生物学 进化生物学
- 细胞生物学 细胞生物学
背景情况:
- 粘膜关联不变T细胞 (MAIT) 具有保存的T细胞受体 (TCR),这意味着关键作用.
- 人类和小鼠MAIT细胞功能的差异掩盖了它们保存的进化特征.
研究的目的:
- 为了研究MAIT细胞的进化保存的功能特征.
- 为了在不同物种的MAIT细胞成熟过程中识别保存的转录事件.
主要方法:
- 采用了与单细胞RNA测序相结合的物种特异性四聚体.
- 分析了6个物种的MAIT细胞发育,涵盖了1.1亿年的进化历史.
主要成果:
- 确定了保留的ZBTB16诱导作为所有研究物种中MAIT细胞成熟的关键标志物.
- 在人类,绵羊,牛和鱼的MAIT细胞中观察到一种共享的类型-1/17转录程序,这表明祖先的特征.
- 注意到人类不变的自然杀手T (iNKT) 细胞也获得了这种类型-1/17程序,这表明与天生的类似T细胞的共同分化途径.
- 记录了动物中独特的1型和17型MAIT细胞子集的发展.
- 揭示了小鼠肠道MAIT细胞获得了一个高度保守的程序,涉及1型,17型,细胞毒性和组织修复基因.
结论:
- MAIT细胞成熟涉及保存的转录事件,包括ZBTB16诱导,跨越广泛的进化范围.
- 天生的类似T细胞,包括MAIT和iNKT细胞,共享共同的分化途径.
- 动物MAIT细胞表现出专门的子集,小鼠肠道MAIT细胞表现出一种独特的,保存的细胞毒性和组织修复程序.
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