玉米转换为微生物酶 通过Phanerochaete chrysosporium生产
Neha Kukreti1, Pravir Kumar1, Rashmi Kataria2
1Department of Biotechnology, Delhi Technological University (DTU), Bawana Road, Delhi, 110042, India.
Applied biochemistry and biotechnology
|December 20, 2023
概括
帕纳罗切特酸可以使用玉米炉高效地产生酶. 固态发酵这种纤维纤维素生物质提供了一种可持续的方法来产生有价值的微生物酶.
科学领域:
- 生物技术是生物技术.
- 环境微生物学 环境微生物学
- 工业酶生产 工业酶生产
背景情况:
- 甲 (Phanerochaete chrysosporium) 是一种白色腐烂的真菌,以分泌细胞外酶而闻名.
- 这些酶对于生物质降解,染料脱色和纸加工等应用至关重要.
- 固态发酵 (SSF) 是一种高效和环保的方法,用于使用纤维素酸材料生产酶.
研究的目的:
- 通过使用SSF,研究玉米作为Phanerochaete chrysosporium酶生产的基质的潜力.
- 评估性预处理对酶产量的影响.
- 为了优化细胞酶和过氧化酶的酶生产参数.
主要方法:
- 使用未经处理和预处理的玉米炉作为Phanerochaete chrysosporium种植的基板.
- 在酶生产中采用固态发酵 (SSF).
- 随着时间的推移量化内葡萄糖酶,外葡萄糖酶和过氧化酶活动.
主要成果:
- 最大内葡萄糖酶产量:121.21U/gds (未经治疗,第9天) 和79.75U/gds (经治疗,第6天).
- 峰值外葡萄糖酶产量:2.46 FPU/ml (未经治疗,第3天) 和0.92 FPU/ml (经过治疗,第6天).
- 最高的过氧化酶产量:5076.81 U/l (未经处理,第6天) 和1127.58 U/l (经处理,第3天).
结论:
- 玉米炉是一个可行的和具有成本效益的基质,用于生产有价值的酶,如纤维酶和过氧化酶.
- 使用Phanerochaete chrysosporium进行固态发酵表明了工业酶生成的巨大潜力.
- 性预处理可能会影响酶产量和生产动力学.
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