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机械力量通过暴露神秘的糖甘结合点来控制疟疾粘合素VAR2CSA的价值

Rita Roessner¹, Nicholas Michelarakis¹, Frauke Gräter^1,2

¹Molecular Biomechanics Group, Heidelberg Institute for Theoretical Studies, Heidelberg, Germany.

PLoS computational biology

|December 20, 2023

概括

机械力量通过改变VAR2CSA蛋白质结构来增强Plasmodium falciparum的附着性. 这种从单价值状态到多价值状态的过渡增加了结合热情,解释了与妊娠相关的疟疾中剪切增强的坚持.

科学领域:

寄生虫学的寄生虫学
结构生物学结构生物学
生物物理学的生物物理.

背景情况:

疟原虫 (Plasmodium falciparum) 引起严重的疟疾,通常通过VAR2CSA粘合物准胎盘.
VAR2CSA调节结合到胎盘里硫酸A (CSA),但切割增强粘附的机制是未知的.

研究的目的:

为了阐明剪切增强VAR2CSA介导粘附背后的分子机制.
研究机械力如何影响VAR2CSA与CSA结合.

主要方法:

分子动力学模拟 (平衡,力探头,基于对接).
在拉力力下模拟VAR2CSA-CSA复合体,模拟血流剪切.

主要成果:

武力应用诱导VAR2CSA打开,暴露了神秘的结合点.
这种形状变化允许多价值CSA分子稳定结合.
机械力量通过将其转移到多价值结合状态来增加VAR2CSA贪.

结论:

机械力量通过强力诱导的过渡到多价值结合来增强VAR2CSA贪.
这种机制解释了Plasmodium falciparum在与怀孕相关的疟疾中的剪切增强粘附性.
这些发现为开发针对VAR2CSA的抗胎盘疟疾疫苗提供了洞察力.

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相关概念视频

Viral Structure

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.