表面化学和反应性离子等离子处理聚乙醇的地形形象的时间变化会改变内皮质化潜力
Ryan A Faase1, Novella M Keeling2,3, Justin S Plaut4
1School of Chemical, Biological, and Environmental Engineering, Oregon State University, 103 Gleeson Hall, Corvallis, Oregon 97331, United States.
ACS applied materials & interfaces
|December 20, 2023
概括
反应性离子等离子治疗增强了内皮细胞对聚乙烯醇血管移植的附着性. 然而,这些对合成血管移植的有益表面修改会随着时间的推移而降解,影响长期的通透性.
科学领域:
- 生物材料科学 生物材料科学
- 表面化学 表面化学
- 组织工程是组织工程.
背景情况:
- 与自身组织相比,直径小的合成血管移植 (<6毫米) 的透度有限,需要改善内皮细胞粘附.
- 聚乙醇 (PVA) 是血管移植的有前途的水凝材料,但需要表面修改以增强细胞融合.
- 内皮细胞粘附对于血管移植在治疗心血管疾病中的成功和通透性至关重要.
研究的目的:
- 在反应性离子等离子体 (RIP) 处理后,对聚乙烯醇 (PVA) 的表面变化进行表征.
- 评估这些表面修改对内皮细胞殖民地形成细胞 (ECFC) 粘附的影响.
- 为了评估RIP诱导的PVA表面变化的稳定性和消散,超过230天的存储.
主要方法:
- 聚乙醇 (PVA) 表面用氧,或反应性离子等离子体 (RIP) 在50和100W处理.
- 使用原子力显微镜 (AFM) 和扫描电子显微镜 (SEM) 分析了表面地形.
- 使用X射线光电子光谱 (XPS) 调查了表面化学,并在48小时后量化了ECFC粘附.
主要成果:
- RIP处理,特别是在更高的射频 (RF) 功率下,增加了表面粗性,并在PVA上引入了带电物种.
- 与未经处理的PVA相比,所有RIP处理都改善了ECFC附着性,取决于前体气体和功率的变化.
- 在标准温度和压力下储存230天后,有益的表面变化和增强的ECFC粘附性显著消散.
结论:
- 反应性离子血 (RIP) 治疗可以有效地增强内皮细胞对聚乙烯醇 (PVA) 血管移植材料的粘附性.
- 更高的射频功率处理导致更明显的表面修饰,有利于细胞附着.
- 这些表面修改的稳定性是有限的,因为它们随着时间的推移而消散,突出显示了对血管移植需要稳定的表面功能化策略的需要.
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