一种可靠的方法,可以通过液态染色学 (液态染色学) 和双重质谱法同时确定人体血清中的八种B族维生素
Yu-Mei Huo1, Shang-Qing Zhang2, Gao-Ping Wu1
1Hangzhou Adicon Clinical Laboratories Co., Ltd, Hangzhou, P. R. China.
Journal of separation science
|December 20, 2023
概括
这项研究提出了一种快速的6分钟方法,可以同时检测人体血清中的8种B族维生素,包括不稳定的科巴胺. 经过验证的试验为基本营养素分析提供了高灵敏度和精度.
科学领域:
- 生物化学 生化学
- 分析化学 分析化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 血清B维生素水平是关键的健康指标.
- 同时检测多种B族维生素,特别是不稳定的甲,由于度低和不稳定,具有挑战性.
- 准确量化B族维生素对于诊断缺陷和监测健康至关重要.
研究的目的:
- 开发和验证人类血清中八种关键维生素B的快速,同时分析方法.
- 为了克服与可胺低度和不稳定性相关的挑战.
- 为例行临床诊断和健康评估提供敏感和精确的方法.
主要方法:
- 使用甲醇进行蛋白质沉,然后进行超高性能液体染色学 (UHPLC) 分离.
- 使用稳定且敏感的Waters Acquity UHPLC HSS T3 列进行染色学分析.
- 根据国际指南 (EMA,FDA,CLSI) 验证方法的准确性,精度和矩阵效应.
主要成果:
- 在6分钟内同时测定了胺,利博弗拉,尼古丁胺,泛多酸,氧酸,生物素,5-甲基四基叶酸和可巴胺.
- 实现了低量化极限,范围为0.04 ng/mL的蓝甲胺到2.0 ng/mL的泛酸.
- 证明了高精度 (1.1% - 13.5%) 和真实性 (0.3% - 13.3%) 与可接受的矩阵效应 (2.6% - 10.4%).
结论:
- 开发的UHPLC方法提供了一种快速,稳定和敏感的方法,用于同时对人血清中的B族维生素进行分析.
- 这种方法有效地解决了量化不稳定和低丰度维生素的挑战,如诺科巴胺.
- 经过验证的试验适用于临床应用,可通过维生素B分析进行有效的健康状况评估.
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