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基于SILAC的精确量化类过敏原tropomyosin在复杂的食物矩阵使用UPLC-MS/MS的精确量化

Yige Wu¹, Kai Yao², Yunjia Yang²

¹National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.

|December 20, 2023

概括

现在可以在复杂的食物中准确检测的食物过敏原tropomyosin. 一种新方法使用全长,标记的热胺标准进行精确的量化,提高食品安全.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
生物化学生物化学

背景情况:

在复杂的食物矩阵中检测微量过敏原是具有挑战性的.
准确量化食物过敏原对于消费者安全和监管合规性至关重要.
现有的方法在复杂的食品环境中可能面临局限性.

研究的目的:

开发和验证一个准确的UPLC-MS/MS定量测试对过敏原tropomyosin.
作为内部标准,确定全长同位素标记的重组热素 (TM-I) 的疗效.
为了应对加工食品中过敏原携带的挑战.

主要方法:

使用超高性能液体色谱-并联质谱法 (UPLC-MS/MS).
使用SILAC技术开发了一种全长的同位素标记的重组热素 (TM-I) 内部标准.
在三个商业加工食品矩阵 (流行食品,汁,surimi) 中验证了测试.

主要成果:

该试验准确量化了从0.2到100 ng/mL的热胺.
平均恢复率在89%至116%之间,精度高 (RSD<12%).
在各种食品中确立了低量限 (1-10μg/g).

结论:

再组合的全长同位素标记蛋白质在过敏原量化方面比标记的精度更高.
开发的方法纠正了消化错误,提高了对食物过敏原检测的可靠性.
这种方法支持通过精确测量过敏原热素来提高食品安全.

关键词:

食物过敏食物过敏再组合的全长同位素标记蛋白质.这就是SILACLAC.托罗皮米奥辛 (Tropomyosin) 是一种可怕的药物.在 UPLC-MS/MS 中使用.

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