一个两阶段的设计增强了氧基膜生物膜反应器中高度C16-基四级化合物的生物降解
Chen-Wei Zheng1, YenJung Sean Lai1, Yi-Hao Luo2
1Biodesign Swette Center for Environmental Biotechnology, Arizona State University, Tempe, AZ, USA.
Water research
|December 20, 2023
概括
一个基于O2的双阶段膜生物膜反应器 (O2-MBfR) 能够有效地生物降解高度的消毒剂六甲基三甲基 (CTAB). 这种方法提高了CTAB的去除率,并支持能够降解CTAB及其代谢物的细菌群落.
科学领域:
- 环境微生物学环境微生物学
- 环境工程环境工程
- 生物技术是生物技术.
背景情况:
- 四级氨化合物 (QAC),如六甲基三甲基 (CTAB),是常见的消毒剂和个人护理成分.
- 在COVID-19大流行期间使用量的增加导致废水中CTAB负载的增加,由于其在高度下对细菌的毒性而带来了挑战.
- 有效的CTAB生物降解需要足够的氧气供应,这一过程由基于O2的膜生物膜反应器 (O2-MBfR) 有效管理.
研究的目的:
- 调查O2-MBfR两阶段系统对高度CTAB的完全生物降解的有效性.
- 为了比较两阶段O2-MBfR与单阶段系统的性能.
- 分析细菌社区结构和基因潜力的变化,以应对CTAB生物降解.
主要方法:
- 实施一个两级O2-MBfR系统,其中包括一个反应堆和一个滞后反应堆.
- 使用高CTAB度 (728毫克/升) 的操作.
- 16S rRNA基因测序和元基因组分析,以评估细菌群体组成和功能基因.
主要成果:
- 两阶段的O2-MBfR实现了超过98%的728 mg/L CTAB的去除.
- 与同等氧供应的单阶段O2-MBfR相比,CTAB的去除率高出70%.
- 细菌群落发生了转变,Pseudomonas和Stenotrophomonas成为主导;像P. alcaligenes和P. aeruginosa这样的特定物种得到了丰富,并且能够降解CTAB代谢物.
- 甲基因组分析表明,由于减少了CTAB的抑制作用,CTAB和代谢物氧化中的代谢物基因被丰富.
结论:
- 两阶段的O2-MBfR是一种高度有效的策略,用于完全生物降解高CTAB度,克服毒性抑制.
- 与单阶段系统相比,这种方法显著提高了CTAB移除效率和速度.
- 该系统培养了一种能够代谢CTAB及其副产品的专业细菌群体,为QAC污染的废水提供可持续的解决方案.
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