来自远鱼 (Boleophthalmus pectinirostristris) 的加勒-9的分子表征和功能研究
Yong-Fei Zhu1, Yi-Fan Hu1, Chang-Hong Li1
1State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Meishan Campus, Ningbo University, Ningbo, 315832, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Meishan Campus, Ningbo University, Ningbo, 315832, China.
Fish & shellfish immunology
|December 20, 2023
概括
泥者素-9 (BpGal-9) 在天生的免疫力中起着关键作用,其N-终端和C-终端域对细菌凝结和对抗Edwardsiella tarda感染的免疫调节有不同的贡献.
科学领域:
- 免疫学 免疫学 免疫学
- 海洋生物学 海洋生物学
- 生物化学 生化学
背景情况:
- 加勒-9是一种重复的双重加勒,对抗微生物感染的先天免疫反应至关重要.
- 了解teleost galectin-9的功能,可以了解鱼类免疫力.
研究的目的:
- 为了识别和表征来自泥者 (Boleophthalmus pectinirostris) 的加勒-9 ,命名为BpGal-9.
- 研究BpGal-9在天生的免疫力中的作用,重点研究其表达,细菌结合和细胞因子调节.
主要方法:
- 对BpGal-9.9的鉴定和序列分析.
- 遗传学分析以确定进化关系.
- 针对Edwardsiella tarda感染的基因表达分析.
- 重组蛋白表达和功能测定 (细菌聚合).
- 细胞因子mRNA表达分析.
主要成果:
- BpGal-9与其他远端物质甲-9.9具有高序列相同性.
- 在E. tarda感染后,BpGal-9表达在各种组织和血细胞中被上调.
- 重组BpGal-9及其C端域表现出广泛的细菌聚合活性.
- BpGal-9优先诱导抗炎细胞因子,而不是促炎细胞因子.
结论:
- BpGal-9的N-终端和C-终端碳水化合物识别域显然有助于其免疫功能.
- BpGal-9是teleosts天生的免疫系统的重要组成部分,参与细菌清除和免疫调节.
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