Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Real Time RT-PCR02:57

Real Time RT-PCR

57.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.3K
Next-generation Sequencing03:00

Next-generation Sequencing

88.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.9K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Sanger Sequencing01:57

Sanger Sequencing

754.5K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Human RNA ligase 1 as a novel regulator of ribosome function and translation under oxidative stress.

Nucleic acids research·2026
Same author

ModiCal: A Targeted Calibration Workflow for Site-Specific m<sup>5</sup>C Validation by Nanopore Direct RNA Sequencing.

ACS chemical biology·2026
Same author

An Alignment Free Framework for Taxonomic Inference From Codon and Codon-Pair Usage.

Ecology and evolution·2026
Same author

tRNA modification landscapes in streptococci: shared losses and clade-specific adaptations.

Open biology·2026
Same author

METTL16 promotes taxane resistance in Triple-Negative Breast Cancer through m <sup>6</sup> A-dependent translational upregulation of ABCB1.

bioRxiv : the preprint server for biology·2026
Same author

2'-O-Methylation maintains ribosome structural and translation integrity.

Molecular cell·2026

相关实验视频

Updated: Jul 7, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

763

在tRNA测序和定量方法的进步.

Nigam H Padhiar1, Upendra Katneni1, Anton A Komar2

  • 1Hemostasis Branch 1, Division of Hemostasis, Office of Plasma Protein Therapeutics, Office of Therapeutic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USA.

Trends in genetics : TIG
|December 20, 2023
PubMed
概括
此摘要是机器生成的。

转移RNA (tRNA) 测序和其他量化方法对于理解细胞过程和开发基于tRNA的治疗方法至关重要. 本综述比较了各种tRNA量化技术,突出了它们的优缺点,以实现可靠的测量.

关键词:
生物信息学是一种生物信息学.混合化 混合化 混合化修改 修改 修改 修改 修改量化量化量化的量化.测序的测序是指测序的测序.这是一个tRNARNA.

更多相关视频

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

9.5K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.0K

相关实验视频

Last Updated: Jul 7, 2025

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

763
Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA
12:35

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

9.5K
2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.0K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物化学 生物化学

背景情况:

  • 转移RNA (tRNA) 测序 (tRNA-seq) 近年来已成为一种至关重要的工具.
  • 精确量化tRNA丰富度,包括修饰和充电物种,对于细胞生理学和疾病研究至关重要.
  • tRNA研究也推动了基于tRNA的新型治疗方法的开发.

研究的目的:

  • 提供各种tRNA量化方法的比较概述.
  • 专注于不同tRNA测量方法的优缺点.
  • 为研究和治疗开发提供可靠的tRNA量化.

主要方法:

  • 对tRNA测序 (tRNA-seq) 方法的审查.
  • 基于下一代测序 (NGS) 的工作流程的比较.
  • 评估基于混合化的方法作为NGS的替代方案.
  • 对计算tRNA修饰和充电/化方法的分析.

主要成果:

  • 存在多种方法来量化tRNA丰富度,修改和充电.
  • 图书馆准备和生物信息学的进步改善了基于NGS的tRNA量化.
  • 基于混合化的方法为特定应用提供了NGS的替代方案.
  • 每种方法都有独特的优点和缺点,可用于可靠的tRNA量化.

结论:

  • 有各种tRNA量化技术可用,每个都有特定的优点和缺点.
  • 了解这些方法是推动tRNA研究在细胞生理学和疾病中的关键.
  • 对量化策略的明智选择对于开发有效的基于tRNA的治疗方法至关重要.