脑脊液中结合细胞的IgM是多发性硬化症的特征,并向铁载体SCARA5的目标
Ilaria Callegari1,2, Johanna Oechtering2, Mika Schneider1
1Department of Biomedicine, University of Basel and University Hospital Basel, Basel 4031, Switzerland.
Brain : a journal of neurology
|December 20, 2023
概括
在多发性硬化症中,内IgM抗体可能通过向SCARA5.5使疾病恶化. 这种结合激活补充体并促进T细胞透,导致中枢神经系统炎症.
科学领域:
- 神经免疫学 神经免疫学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 在多发性硬化症 (MS) 中,内IgM的产生与更严重的疾病过程相关.
- 自动反应性IgM在MS发病过程中的致病作用尚不完全理解.
研究的目的:
- 为了研究自身反应性IgM在多发性硬化症中的病原性相关性.
- 在MS患者中确定内IgM准的特定自身抗原.
主要方法:
- 从MS患者的脑脊液 (CSF) 的查和对照对IgM与神经元和天体细胞衍生的细胞的结合.
- 测序B细胞转录组以产生重组单克隆IgM抗体.
- 使用免疫沉,质谱和转录组分析进行抗原鉴定.
- 在实验性自身免疫脑膜炎 (EAE) 模型中评估SCARA5抗体效应.
主要成果:
- 结合IgM与神经外皮细胞系的IgM突出的MS捐赠者.
- 产生了五种细胞结合性重组IgM抗体;一个激活补充.
- SCARA5被确定为补充激活IgM的抗原.
- 在EAE模型中,SCARA5抗体的内注射增加了T细胞透率.
结论:
- 内IgM可能通过向SCARA5.5等细胞表面抗原,导致MS中中枢神经系统炎症.
- 通过补体激活或免疫细胞迁移,SCARA5反应性IgM可能会促进神经炎症.
相关概念视频
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