裸鼠的成年干细胞活性用于长期组织维护
Shamir Montazid1, Sheila Bandyopadhyay2, Daniel W Hart3
1Nuffield Department of Clinical Laboratory Sciences, Radcliffe Department of Medicine, University of Oxford, Oxford, OX3 9DU, UK.
Nature communications
|December 20, 2023
概括
最长寿的动物裸鼠 (NMRs) 显示肠道干细胞分裂速度较慢,粘膜功能增强,为衰老和寿命提供了洞察力.
科学领域:
- 老年学是一门学科.
- 干细胞生物学 干细胞生物学
- 比较生理学比较生理学
背景情况:
- 裸鼠 (NMR) 是最长寿的动物,因此它是研究衰老和寿命的模型生物.
- 了解成年干细胞 (ASC) 活动如何随着寿命的延长而保持,对于衰老研究至关重要.
研究的目的:
- 研究与其特殊寿命相关的NMR中独特的肠干细胞 (ISC) 行为和组织功能背后的机制.
- 将NMR中的ISC动态与短命动物和人类的ISC动态进行比较.
主要方法:
- 在体内使用累积氧氨 (BrdU) 标签来追踪肠道ASC (Lgr5+).
- 采用定量成像来分析密码基层 (Lgr5+CBC) 的Lgr5+细胞的增殖和细胞周期动态.
主要成果:
- 核磁共振具有扩大的Lgr5+肠干细胞池.
- 虽然NMR Lgr5+CBC细胞的分裂速度比小鼠更慢,但与人类的LGR5+CBC细胞的生长率相似.
- 通过延长G1和/或G2的停止,NMRISCs延缓细胞分裂,而不是进入静止状态 (G0).
- 核磁共振具有较高比例的分化细胞,增强肠道粘膜的保护和对光质失衡的反应能力.
结论:
- 在NMR中肠道干细胞的独特细胞循环调节有助于它们的寿命和组织维护.
- 在NMR中增强的肠粘膜功能提供了保护和有效检测光不平衡.
- 核磁共振作为一种有价值的模型,用于理解干细胞适应长寿和促进健康衰老的潜在策略.
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