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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Purification of Transcripts and Metabolites from Drosophila Heads

Purification of Transcripts and Metabolites from Drosophila Heads

Published on: March 15, 2013

大脑表体转录学研究和翻译机会的进步.

Feng Zhang¹, Valentina V Ignatova¹, Guo-Li Ming^2,3,4,5

¹Department of Neuroscience and Mahoney Institute for Neurosciences, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Molecular psychiatry

|December 20, 2023

概括

经转录学,即对RNA修饰的研究,对大脑发育和功能至关重要. 这些修改为神经系统疾病和mRNA疫苗的进步提供了有希望的治疗策略.

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Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus

Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus

Published on: March 8, 2015

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

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相关实验视频

Last Updated: Jul 7, 2025

Purification of Transcripts and Metabolites from Drosophila Heads

Purification of Transcripts and Metabolites from Drosophila Heads

Published on: March 15, 2013

Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus

Analysis of Gene Expression Changes in the Rat Hippocampus After Deep Brain Stimulation of the Anterior Thalamic Nucleus

Published on: March 8, 2015

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Published on: May 22, 2021

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

经转录组学涉及RNA的化学修饰,调节RNA代谢和生物过程.
RNA的修改对神经发育,大脑功能和大脑疾病的病变产生至关重要.
在COVID-19 mRNA疫苗中RNA修饰的成功突出了它们的临床潜力.

研究的目的:

在动物模型中提供RNA修饰和表体转录学研究的概述.
阐明RNA修饰在哺乳动物神经发生,质发生,突触形成和大脑功能中的作用.
强调RNA修饰在人类大脑疾病中的参与,并讨论转化机会.

主要方法:

综述了近期表体转录学研究的进展.
利用动物模型研究神经过程中的RNA修饰作用.
分析RNA修饰在各种脑部疾病的发病过程中的参与.

主要成果:

RNA的修改极大地调节了哺乳动物的神经发生,质发生,突触形成和整体大脑功能.
RNA修饰途径在神经发育,精神病,神经退行性疾病和脑瘤的发病过程中至关重要.
体转录调节器与各种人类大脑病理的进展有关.

结论:

RNA修改为治疗大脑疾病提供了显著的转化机会,包括生物标志物开发和向治疗.
应用包括药物开发,用于表体转录体路径的基因疗法,以及基于mRNA的疫苗/疗法.
解决目前表体转录学研究的局限性对于广泛的临床应用至关重要.