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缺血性中风血栓透性与可区分的蛋白质组特征和易受ADAMTS13增强血栓溶解的敏感性有关
Derrek Schartz1,2, Sajal Medha K Akkipeddi2, Redi Rahmani2
1From the Department of Imaging Sciences (D.S., L.W.), University of Rochester Medical Center, Rochester, New York derrek_schartz@urmc.rochester.edu.
AJNR. American journal of neuroradiology
|December 20, 2023
概括
血栓透性,即凝块透性的度量,与不同的蛋白质组成有关,特别是涉及血小板和免疫细胞. 高透性血栓更容易受到特定的血栓消毒疗法的影响.
科学领域:
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
- 医疗成像医学成像
背景情况:
- 透射性,定义为对比后内动脉封闭性血栓的差异性CT衰减,与临床结果和本病理学有关.
- 血栓穿透度和蛋白质组合之间的关系在很大程度上仍未被探索.
研究的目的:
- 为了研究血栓穿透度与内静脉封闭性血栓中蛋白质组合之间的关联.
- 探索透性对血栓敏感性对血栓溶解剂的影响.
主要方法:
- 量化质谱法用于分析59个检索到的瘤的蛋白质组概况.
- 血栓被分为高通透度 (≥11 HU) 或低通透度 (<11 HU),并将通透度分析为连续变量.
- 使用tPA,脱氧核糖酶和ADAMTS13.2进行了ex vivo血栓溶解试验.
主要成果:
- 总共有2790种不同的蛋白质被确定,其中的透性与独特的蛋白质特征有关.
- 穿透性血栓显示了巨细胞标记物 (CD14) 和血红蛋白子单元 ζ. 的枯竭.
- 参与血小板细胞骨架重塑和颗粒分泌的蛋白质与透性增加有关;单细胞/巨细胞蛋白质在透性栓塞中被耗尽.
- 穿透的血块更容易受到ADAMTS13增强的tPA血栓溶解,但不是脱氧核糖酶消化.
结论:
- 血栓透性与复杂的蛋白质特征有关,包括血小板相关蛋白质的差异丰富性和高透性血栓中单细胞/巨细胞蛋白质的枯竭.
- 这些蛋白质组的差异可能解释了高度通透的血栓对ADAMTS13增强血栓溶解的易感性增加.
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