相关实验视频

Updated: Jul 7, 2025

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
14:12

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

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光相关谱作为一种多功能方法,以确定单分子灵敏度的aptamer-protein相互作用.

David Porciani1,2, Manuela Maria Alampi3, Stefania Abbruzzetti3

  • 1MU Bond Life Sciences Center, University of Missouri-Columbia, 1201 Rollins Street, Columbia, Missouri 65211-7310, United States.

Analytical chemistry
|December 21, 2023
PubMed
概括

光相关谱法 (FCS) 提供单分子灵敏度,用于胺蛋白结合分析. 这种方法准确地量化了阿普坦酶结合动力学和特异性,即使在复杂的生物环境中也是如此.

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A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts08:43

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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