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SATORI:用于诊断病毒感染的无放大数字RNA检测方法

Tatsuya Iida1, Hajime Shinoda1, Rikiya Watanabe1

  • 1Molecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN, Wako, Saitama 351-0198, Japan.

Biophysics and physicobiology
|December 21, 2023
PubMed
概括

一种新的无放大数字RNA检测方法SATORI可以在9分钟内快速识别单分子病毒基因. 这种多功能诊断平台,包括自动化和便携式系统,有望改善COVID-19等传染病的治疗点检测.

关键词:
这就是CRISPR-Cas13a.病毒RNARNA病毒RNA病毒自动化自动化自动化自动化数字生物分析数字生物分析遗传测试 遗传测试 遗传测试是一种

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...