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在没有RORγt表达的情况下,对全身疫苗的口服耐受性在调节性T细胞中保持不变

Nicole B Potchen^1,2, Andrew M F Johnson¹, Kevin Hager¹

¹Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.

|December 21, 2023

概括

表达RORγt的调节性T细胞 (Tregs) 不必对口服抗原暴露产生耐受性并影响全身疫苗反应. 这一发现影响了疫苗疗效研究.

科学领域:

免疫学免疫学免疫学
疫苗学疫苗学疫苗学

背景情况:

疫苗的有效性因个体免疫反应而有所不同.
通过调节性T细胞 (Tregs) 介导的胃肠道免疫耐受性可能会降低疫苗的有效性.
在口服抗原暴露后,RORγt+ Tregs在系统性疫苗反应中的特定作用尚不清楚.

研究的目的:

研究RORγt表达调节性T细胞 (Tregs) 在调节口服抗原诱导耐受性的作用.
确定是否需要RORγt+ Tregs来调节口服抗原预暴露后的全身疫苗反应.

主要方法:

使用条件淘汰鼠标模型,专门删除Tregs.中的RORγt.
在全身免疫接种之前,口服服用卵素 (OVA).
在Tregs.表达RORγt和没有RORγt的小鼠中评估疫苗诱导的抗体反应.
研究了不同辅助剂对这些反应的影响.

主要成果:

在Tregs中缺乏RORγt表达的小鼠在口服OVA暴露后与对照组相比,表现出类似的疫苗诱导抗体反应.
在Tregs中存在或缺乏RORγt并没有显著改变对口服抗原的系统性耐受性诱导.
辅助剂类型影响了研究结果,表明了复杂的相互作用.

结论:

不需要RORγt+ Tregs来诱导对口服抗原的全身耐受性.
在口服抗原暴露后,RORγt+ Tregs在调节全身疫苗反应中的作用并不重要.
需要进一步的研究来了解口服耐受性和疫苗反应的细微机制,可能涉及其他Treg子集或因素.

关键词:

细胞生物学细胞生物学免疫反应的免疫反应.免疫学免疫学免疫学

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