工程 pine endophytic Bacillus toyonensis 具有 nematocidal 和殖民能力,用于控制松病的控制
Dongzhen Li1,2, Yongxia Li1,2, Xuan Wang1,2
1Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.
Frontiers in microbiology
|December 21, 2023
概括
研究人员设计了一种Bacillus toyonensis菌株来控制松树线虫 (PWN),这是松病的原因. 工程细菌有效地殖民松树苗并保护它们免受感染,提供了一种可持续的害虫管理策略.
科学领域:
- 植物病理学 植物病理学
- 微生物生态学 微生物生态学
- 生物控制剂 生物控制剂
背景情况:
- 松树线虫 (PWN) 在欧亚大陆森林中引起破坏性松病 (PWD).
- 松树是一种侵入性的隔离害虫,在全球范围内威胁着针叶树种类.
- 用植物相关的微生物控制线虫是一种可持续的害虫防治策略,但找到合适的细菌是具有挑战性的.
研究的目的:
- 隔离和工程松相关细菌与nematocidal活动和殖民能力.
- 评估工程细菌在松树苗中对松树线虫感染的疗效.
主要方法:
- 查松树相关细菌对内部松树环境的耐受性.
- 工程一个Bacillus toyonensis菌株 (Bxy19) 对于nematocidal活动 (Cry6Aa蛋白).
- 通过光和温室实验追踪工程菌株的分散和殖民.
主要成果:
- 隔离的Bacillus toyonensis Bxy19具有很高的抗压能力.
- 构造的工程菌株Bxy19P3C6具有无毒杀菌活性.
- 证明Bxy19GFP在针头中分散,并殖民血管捆和气管,持续超过50天.
- 温室测试显示,Pinus massoniana幼苗对线虫感染有显著的保护.
结论:
- 工程化Bacillus toyonensis有效地殖民松树组织并持续存在.
- 这项研究提出了一个有前途的生物控制策略,使用工程细菌和Bt蛋白来对抗线虫.
- 这些发现有助于更好地理解用于害虫控制的松树中的细菌殖民.
关键词:
细菌杆菌是一种Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt Bt 这是一个比没有的没有的没有的没有的细菌殖民化的细菌殖民工程细菌就是工程细菌.松木线虫 松木线虫与植物相关的微生物.更多相关视频
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