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识别和功能预测与连接组织疾病相关的长非编码RNA和mRNA的连接组织疾病相关的间歇性肺病

Fei Dai^1,2, Yixi He^1,2, Tianyi Lei^1,2

¹Research Center of Hyperuricemia and Gout, the Afiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China.

Rheumatology and immunology research

|December 21, 2023

概括

这项研究揭示了连接组织疾病相关的间歇性肺病 (CTD-ILD) 患者中明显的长非编码RNA (lncRNA) 概况. 这些发现为CTD-ILD病原体和潜在的诊断标记提供了新的见解.

科学领域:

免疫学免疫学免疫学
基因组学就是基因组学.
肺部病理学肺部病理学

背景情况:

长非编码RNAs (lncRNAs) 越来越多地被认为在风湿性免疫疾病中的作用.
然而,它们在连接组织疾病相关的间歇性肺病 (CTD-ILD) 中的具体参与仍未得到充分研究.
了解lncRNA的表达对于阐明CTD-ILD的病原性至关重要.

研究的目的:

研究CTD-ILD患者外周血液单核细胞 (PBMC) 中的lncRNA和信使RNA (mRNA) 的表达特征.
确定CTD-ILD涉及的潜在分子机制和信号通路,特别是系统性硬化症 (SSc) -ILD和类风湿性关节炎 (RA) -ILD.
探索CTD-ILD的新诊断和治疗目标.

主要方法:

进行了LncRNA微阵列分析,以比较CTD-ILD和非ILD (CTD-NILD) 组之间的基因表达.
生物信息学分析确定了差异表达的基因和相关的生物过程/信号通路.
定量逆转录PCR (qRT-PCR) 在更大的SSc和RA患者队列中验证了特定lncRNA和mRNA的表达水平.

主要成果:

微阵列分析确定了240个差异表达的lncRNA和218个差异表达的mRNA.
生物信息学分析强调了NF-kappa B,IL-17和B细胞受体信号通路的参与.
在ILD患者中,lncRNA ENST00000604692的上调显著,而T311354和阿基纳酶-1在SSc中比RA患者高.

结论:

这项研究确定了CTD-ILD患者的PBMC中明显的lncRNA表达特征.
这些发现表明,特定的信号通路与CTD-ILD的病原发生有关.
已识别的lncRNAs可以作为CTD-ILD的诊断和治疗的潜在生物标志物.

关键词:

与结合组织疾病相关的间歇性肺病.功能分析是一种功能分析.长非编码RNA是什么意思微阵列分析分析的方法

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lncRNA - Long Non-coding RNAs

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