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在肺高血压患者中进行全基因组微RNA测序分析

Shi Chen^1,2, Jinnan Zhong², Bingzhu Hu²

¹Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China.

Frontiers in genetics

|December 21, 2023

概括

研究人员在周围血液中确定了四种微RNA (miRNA),这些微RNA可以作为肺动脉高血压 (PAH) 的诊断生物标志物. 这一发现为早期检测和改善这种心血管和呼吸系统疾病的管理提供了潜力.

科学领域:

生物化学生物化学
遗传学遗传学是一个
心脏病学心脏病学

背景情况:

肺高血压 (PH) 是一种复杂的疾病,影响心血管和呼吸系统.
微RNA (miRNA) 越来越被认为是疾病发病过程中的关键信号分子.
目前缺乏可靠的PH诊断和预后生物标志物.

研究的目的:

为了确定新的微RNA (miRNA) 生物标志物用于诊断肺高血压 (PH).
研究循环miRNAs作为PH的非侵入性诊断工具的潜力.

主要方法:

来自PH患者和健康对照的外周血液样本的全基因组miRNA测序和转录组分析.
对差异表达的miRNA进行查.
候选miRNA生物标记物的验证.

主要成果:

在PH患者中鉴定出136种差异表达的miRNA.
验证了四种特定的miRNAs (hsa-miR-1304-3p,hsa-miR-490-3p,hsa-miR-11400和hsa-miR-31-5p) 作为肺动脉高血压的潜在临床诊断生物标志物.
这些miRNA显示出区分PH患者和健康个体的希望.

结论:

已识别的miRNAs代表了肺动脉高血压的潜在新型诊断生物标志物.
这些发现为了解特定miRNA在PH中的作用奠定了基础.
进一步研究这些miRNA在PH中的机械作用是有必要的.

关键词:

临床诊断生物标志物不同的表达方式,不同的表达方式.这是一个微型RNA.肺高血压是一种肺高血压.转录基因组测序的转录基因组测序

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相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...