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Lucía Barrado-Gil¹, Isabel García-Dorival¹, Inmaculada Galindo¹

¹Departmento Biotecnología, INIA-CSIC, Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain.

Frontiers in cellular and infection microbiology

|December 21, 2023

概括

非洲猪瘟病毒 (ASFV) 感染依赖于细胞膜重塑,涉及运输 (ESCRT) 蛋白VPS4A和ALIX所需的内体组分复合体. 它们的操纵会影响ASFV复制和病毒标位.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

非洲猪瘟病毒 (ASFV) 需要细胞内细胞通路和膜重塑以感染.
运输 (ESCRT) 机器所需的内体组分复合体调节了膜动态,并被各种病毒利用.
辅助蛋白VPS4A和ALIX是ESCRT通路的关键组成部分.

研究的目的:

研究ESCRT相关蛋白VPS4A和ALIX在ASFV感染中的作用.
确定VPS4A和ALIX操纵对ASFV复制和病毒产生的影响.
为了探索 lysobisphosphatidic 酸 (LBPA) 在 ASFV 感染中的参与.

主要方法:

通过shRNA介导的VPS4A和ALIX的淘汰.
对ASFV复制和病毒标位的分析.
对HRS和ALIX表达水平的评估.
功能阻断LBPA. 的功能阻断.

主要成果:

VPS4A敲击降低了ASFV复制和病毒标位,增强了HRS表达.
ASFV感染增加了HRS和ALIX的表达,但VPS4A的枯竭阻止了HRS的上调.
艾利克斯沉默会影响晚期ASFV感染,复制和生产.
阻断LBPA降低了ASFV在早期和晚期阶段的感染力.

结论:

VPS4A和ALIX在ASFV生命周期中发挥着至关重要的作用.
与ESCRT相关的蛋白质对于ASFV感染至关重要.
LBPA与ASFV感染性有关,影响病毒周期的多个阶段.

关键词:

在ASFV中,ASFV是ASFV.非洲猪瘟病毒病毒阿里克斯·阿里克斯 (Alix) 是一个这是ESCRT的特色.LBPA LBPA LBPA LBPA LBPA LBPA LBPA LBPA LBPA LBPA LBPA LBPA LBPA LB 在NPC1中,NPC1是NPC1.在Vps4中使用Vps4.多个体的多个体体的身体.

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