对幻灯片扫描仪,扫描设置和细胞准备剂进行比较评估,用于数字尿细胞学
Jen-Fan Hang1,2, Yen-Chuan Ou3, Wei-Lei Yang4
1Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.
Journal of pathology informatics
|December 21, 2023
概括
为细胞学幻灯片优化全幻灯片成像 (WSI) 需要先进的焦点模式. 半自动或手动对焦模式实现100%的扫描成功,对于精确的AI分析异常细胞至关重要.
科学领域:
- 数字病理学数字病理学
- 细胞病理学 细胞病理学
- 医学成像医学成像
背景情况:
- 3D细胞学幻灯片的数字图像采集带来了重点挑战.
- 本研究评估了使用两个扫描仪和三个焦点模式的全幻灯片图像 (WSI) 性能.
研究的目的:
- 评估不同焦点模式和扫描仪对细胞学WSI质量的影响.
- 为了确定最佳的扫描参数,以精确的基于AI的非典型细胞分析.
主要方法:
- 用Cytospin和ThinPrep方法准备尿癌尿样.
- 使用Leica和Hamamatsu扫描仪生成了WSI,具有默认,半自动和手动对焦模式,包括Z堆.
- 性能指标包括扫描成功率,AI推断的细胞计数,覆盖率,扫描时间和文件大小.
主要成果:
- 半自动和手动对焦模式提高了扫描成功率,达到92.9-100%.
- 焦点模式没有显著改变AI推断的非典型细胞数量或覆盖率.
- 扫描器类型,细胞准备和Z堆叠影响了细胞计数,覆盖范围,扫描时间和文件大小.
结论:
- 建议采用半自动或手动对焦模式,以实现最佳扫描成功 (高达100%).
- 为了充分覆盖非典型细胞 (80%),至少需要9层Z堆叠.
- 虽然Z-stacking可以改善AI分析,但它增加了扫描时间和文件大小.
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