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在生长受限后代的脏中印制的基因变化可能由长非编码RNA介导

Thu N A Doan^1,2, James M Cowley¹, Aaron L Phillips¹

¹School of Agriculture, Food and Wine, & Waite Research Institute, University of Adelaide, Adelaide, South Australia, Australia.

|December 21, 2023

概括

怀孕期间的环境侮辱会改变生长受限后代的表观遗传机制. 这项研究显示了长非编码RNA Kcnq1ot1表达和DNA甲基化在生长受限制的老鼠脏中的变化,这表明它在发育编程中发挥了作用.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
发展生物学发展生物学
基因组学就是基因组学.

背景情况:

变化的表观遗传机制与暴露于母亲环境侮辱的后代的生长限制有关.
之前的研究报告了DNA甲基转移酶Dnmt3a和Cdkn1c和Kcnq1印记基因在生长受限制的老鼠脏中的变化.

研究的目的:

调查生长受限制后代改变印制基因表达背后的机制.
检查KvDMR1印记控制区域 (ICR) 的DNA甲基化及其与Kcnq1ot1表达的关系.

主要方法:

在大鼠脏中研究了KvDMR1 ICR的DNA甲基化.
与对照人群相比,在生长受限制的后代中测量了Kcnq1ot1表达.
分析了Kcnq1ot1,Kcnq1和Cdkn1c表达和DNA甲基化水平之间的相关性.

主要成果:

在出生后的第1天,Kcnq1ot1表达在生长受限后代下降了51%.
在生长受限制的胎儿中观察到Kcnq1ot1和Kcnq1表达之间的负相关性.
在KvDMR1 ICR中的一个CpG位点发现甲基化下降11.25%.

结论:

长非编码RNAs,如Kcnq1ot1,可能在调解生长受限后代组织中观察到的表观遗传变化中发挥作用.
表观遗传失调,包括改变的DNA甲基化和lncRNA表达,有助于对子宫内侮辱的反应的发育编程.

关键词:

通过DNA甲基化.宫内生长限制宫内生长限制表观遗传机制的表观遗传机制长非编码RNA是什么意思这是子宫胎盘缺陷.

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