使用人类衍生的THP-1细胞激活测试选与皮肤接触产品的过敏性潜力
Yao Pei1,2, Jing Ren1,2, Hongrui Zhang1,2
1State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Environmental science & technology
|December 21, 2023
概括
选与皮肤接触产品的过敏潜力至关重要. 一种使用人工汗水和人类细胞系激活试验 (h-CLAT) 的新方法成功地在77种测试物品中的26种,包括可穿戴设备和化品中确定了过敏原.
科学领域:
- 皮肤病学 皮肤病学
- 毒理学 毒理学 毒理学
- 免疫学 免疫学 免疫学
背景情况:
- 过敏性接触性皮肤炎 (ACD) 是一个重要的公共卫生问题,与广泛的皮肤接触产品有关.
- 需要有效的选方法来评估消费品 (如可穿戴设备,护肤品和护发产品) 的敏感化潜力.
研究的目的:
- 开发和验证一种可靠的体外法,用于选各种皮肤接触产品的皮肤敏感化潜力.
- 确定特定的产品类别并量化其中的过敏原的流行程度.
主要方法:
- 使用了一种优化的体外人体细胞系激活试验 (h-CLAT).
- 77种与皮肤接触的产品 (腕表带,护肤品,护发品,手套) 使用中性人造汗水提取,以模仿真实世界的暴露.
- 提取物使用h-CLAT进行了测试,测量了THP-1细胞上的CD54和CD86表达.
主要成果:
- 在77种产品中,26种产品的过敏潜力测试呈阳性.
- 腕表带 (9/26) 和手套 (9/26) 显示过敏原的流行率最高,其次是护肤品 (6/26) 和护发产品 (2/26).
- 过敏反应主要与CD54表达诱导有关,CD54 / CD86水平因细胞活性的影响而变化.
结论:
- 结合中性人造汗液提取与h-CLAT查的强有力的方法已经建立起来,用于评估消费品中的皮肤过敏潜力.
- 这种方法有助于识别日常用品中的潜在过敏原,有助于提高产品的安全性和质量.
- 这些发现强调了需要对与皮肤接触的产品进行严格的测试,以减轻ACD对公共健康的影响.
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