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作为癌症生物标志物的RUNX1甲基化,可以区分乳头甲状腺癌与良性甲状腺结节
Junjie Li1, Yifei Yin2, Haixia Huang1
1Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing, 210000, China.
Epigenomics
|December 21, 2023
概括
调查甲状腺结核中的DNA甲基化发现RUNX1低甲基化与乳头甲状腺癌 (PTC) 有关. 这一发现表明RUNX1甲基化是诊断恶性甲状腺结节的潜在生物标志物.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 准确诊断甲状腺结节是具有挑战性的,特别是当活检产生不确定的结果时.
- 区分乳头甲状腺癌 (PTC) 和良性甲状腺结节 (BTNs) 需要可靠的生物标志物.
- 异常的DNA甲基化模式与各种癌症有关,包括甲状腺恶性瘤.
研究的目的:
- 与良性甲状腺结节 (BTNs) 相比,识别和描述早期乳头甲状腺癌 (PTC) 的异常DNA甲基化特征.
- 评估已识别的甲基化标记物作为甲状腺癌诊断生物标记物的潜力.
主要方法:
- 在早期的PTC和BTN组织样本上使用850K阵列和RNA测序进行全基因组分析.
- 在两个独立的病例控制研究中使用质谱测量验证候选基因.
- 统计分析以评估已识别的甲基化模式的诊断准确性.
主要成果:
- 与BTN相比,在PTC组织中发现了RUNX1基因显著的低甲基化模式.
- 识别的RUNX1低甲基化在独立验证队列中得到了一致的验证.
- RUNX1甲基化在区分早期PTC和BTN方面表现出很好的准确性,在年轻女性中显著有效.
结论:
- 在RUNX1低甲基化和乳头甲状腺癌存在之间存在显著的关联.
- RUNX1甲基化作为一种有前途的潜在生物标志物,用于对恶性甲状腺结节的伴侣诊断.
- 对RUNX1甲基化的进一步研究可能会导致对未确定的甲状腺结节的改善诊断策略.
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