OctoShaker:用于细胞和有机体研究的多功能机器人生物机械动器
Yan Huang1, Soojung Lee1, Wenhao Liu1
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA.
The Review of scientific instruments
|December 21, 2023
概括
一个新的机器人仪器OctoShaker,用于生物研究,产生复杂的机械力. 这种工具模仿了体内条件,影响细胞和器官的发育,以进行先进的生物力学研究.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 机器人技术 机器人技术 机器人技术
背景情况:
- 机械力量是细胞发育和功能的关键调节者.
- 现有的生物摇技术提供了有限的运动模式,不足以模拟体内条件.
- 了解生物物理线索需要精确的机械刺激工具.
研究的目的:
- 介绍OctoShaker,一个多功能机器人仪器,用于产生复杂的机械力.
- 编程和展示OctoShaker模拟复杂的体内血液动力学的能力.
- 调查程序化机械运动对微粒子分布和生物样本的影响.
主要方法:
- 开发了OctoShaker,一个多动作机器人仪器.
- 编程循环对流运动,用于机械刺激.
- 在96孔板,HeLa细胞和有机体中测试微粒分布.
主要成果:
- OctoShaker成功地产生了编程的圆形对流运动.
- 在微粒子分布,HeLa细胞和有机体中观察到独特的结果模式.
- 证明了仪器在复杂的生物力学研究中的潜力.
结论:
- OctoShaker为生物研究中的动态机械力产生提供了一个多功能平台.
- 该仪器的功能扩展到细胞和有机体研究中的各种应用.
- 预计OctoShaker的开源传播将推进生物力学研究.
相关概念视频
01:32Tissue Homogenization and Cell Lysis
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
01:18Bioreactor Controls-II
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...