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穿透细胞的-石自行车

Saan Voss^1,2, Liam D Adair^3,4, Katharina Achazi⁵

¹Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia.

Angewandte Chemie (International ed. in English)

|December 21, 2023

概括

我们开发了新的-双自行车作为细胞透 (CPPs),用于增强载荷输送到人体细胞中. 这些双循环CPP的细胞吸收率明显高于传统的CPP,如TAT或八氨酸.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
纳米技术纳米技术

背景情况:

细胞透 (CPPs) 对于细胞内传递至关重要.
现有的CPP如TAT和八素在效率和充电要求方面存在局限性.

研究的目的:

开发和表征基于-石自行车的新型CPP.
为了评估这些新的CPP与传统的相比,其细胞吸收效率.

主要方法:

从商业前体中合成-双自行车.
活细胞共聚焦显微镜用光标记.
感应合等离子体质谱法 (ICP-MS) 用于量化细胞内石.

主要成果:

证明了-双自行车的细胞进入人体细胞.
与TAT和octaarginine相比,实现了显著更高的细胞吸收 (超过一个数量级).
使用ICP-MS确认了细胞内斯木的存在,验证了另一种吸收分析方法.

结论:

酸比斯穆特自行车代表了一类新型高效的CPP.
由石诱导的自行车化 (III) 极大地增强了细胞吸收.
细胞吸收机制依赖于能量,可能涉及巨细胞.

关键词:

自行车自行车自行车比斯穆特 (Bismuth) 是一种天然矿物.细胞细胞细胞光是一种光效应.酸是一种酸.

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