在犬类非对称化过程中,表观基因组素H3化标记区分单价和双价形成染色体
Radka Kalfusová1, Veit Herklotz2, Katrin Kumke3
1Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 00 Brno, Czech Republic.
Annals of botany
|December 21, 2023
概括
多格罗斯半转化涉及双价和单价染色体. 降低基因组H3酸化在单等价物中导致它们的低凝结和在雄性半变异过程中过早的姐妹染色体分裂.
科学领域:
- 植物遗传学 植物遗传学
- 分子细胞遗传学 分子细胞遗传学
- 生殖生物学 生殖生物学
背景情况:
- 狗 (Rosa科) 是一种植物. 狗类 (Caninae) 通常是五体状的,有35个染色体.
- 它们表现出不对称的半变异,具有双价和单价染色体.
- 单价染色体在显微胞代过程中被消除,而双价染色体是通过性传播传播的.
研究的目的:
- 为了研究在多格洛斯半溶性中基因组H3酸化模式.
- 为了区分双价和单价形成的染色体.
- 了解狗中介性染色体凝聚和分离的调节.
主要方法:
- 免疫组织化学和分子细胞遗传学用于罗莎犬和罗莎鲁比金诺萨的雄性半导体变异.
- 分析了质子修饰模式 (H3S10p,H3S28p,H3T3p,H3K4me3,H3K27me3) 和α-素.
- 用一个18S rDNA探针进行光现场杂交.
主要成果:
- 单价染色体经历了方程分裂,而双价染色体则正常分离.
- 双对应染色体显示出强烈的H3酸化,特别是在近位区域.
- 单价物具有较弱的H3酸化,主要在邻近区域之外,与低凝结相关.
结论:
- 单等价物中没有配对/再组合,可能会损害组织素H3酸化.
- 这可能会影响介质特异性的凝聚力负荷和姐妹染色体凝聚力.
- 不稳定的凝聚力可能导致姐妹染色体在第一个介质分裂中过早分裂.
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