黄类化合物通过ROS清理在植物Atriplex canescens中参与盐分耐受性
Shan Feng1, Yu-Ting Yao1, Bei-Bei Wang1
1State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730000, China.
Plant cell reports
|December 21, 2023
概括
黄类化合物在盐水条件下在植物中积累,增强盐和透应激耐受性. 过度表达AcCHI基因可以促进黄酸的产生,通过维持活性氧物种 (ROS) 恒温,改善压力下的植物存活率和生物量.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 阿特里普莱克斯 (Atriplex canescens) 是一种适应盐水环境的半植物灌木.
- 之前的研究表明,在NACl处理下,A. canescens中的黄类生物合成基因上调.
- 黄素在A. canescens盐度反应中的作用尚不清楚.
研究的目的:
- 为了调查黄类药物在A. canescens对盐应激反应中的参与.
- 确定AcCHI基因在黄类生物合成和应激耐受性中的功能.
主要方法:
- 在NACl处理下对A. canescens的黄类积累和关键酶基因表达 (AcCHS,AcCHI,AcF3H) 的分析.
- 转基因Arabidopsis thaliana产生过度表达AcCHI的基因.
- 评估转基因A. thaliana的盐和透应激耐受性,包括生物质,H2O2含量和血透性.
主要成果:
- 在A. canescens的叶子和根在100和300mM NaCl.以下,类积累显著增加.
- 通过NaCl处理诱导了AcCHS,AcCHI和AcF3H基因的表达.
- 转基因Arabidopsis thaliana中的AcCHI过度表达增强了黄类生物合成,导致盐和透应激耐受性得到改善,H2O2水平降低,与野生类型植物相比,血透度降低.
结论:
- 黄类化合物在A. canescens的盐应激反应中发挥着至关重要的作用,主要是通过反应性氧物种 (ROS) 清理.
- 该AcCHI基因是黄类生物合成的关键参与者,并赋予显著的盐和透应激耐受性.
- 在料和作物植物中,AcCHI具有提高压力耐受性的潜力.
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