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比例光和裸眼可视化的双通道传感策略,用于Cu2+和性酸酶活性测定

Jing Zhu¹, Xinyu Li²

¹College of Chemistry and Chemical Engineering, Qufu Normal University, Qufu Shandong, 273165, People's Republic of China. zhujing4452830@126.com.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

|December 21, 2023

概括

这项研究提出了一种新的双模式传感器,用于检测铜离子 (Cu2+) 和酸酶 (ALP). 该传感器利用智能手机辅助的视觉检测,为疾病诊断和药物查提供了潜在的工具.

科学领域:

分析化学分析化学
生物医学传感传感器
光光谱学光光谱学

背景情况:

铜离子 (Cu2+) 和性酸酶 (ALP) 是各种疾病的关键生物标志物.
准确而敏感的检测方法对于疾病诊断和监测至关重要.

研究的目的:

开发一种新的二进制比率-光和智能手机辅助视觉传感器战略.
为了使Cu²⁺和ALP活动的敏感和同时检测.

主要方法:

设计了一种基于4'-aminomethyl-4, 5', 8-trimethylpsoralen (AMT) 和o-phenylenediamine氧化的双排放比光传感器.
利用了AMT的光灭和对Cu²⁺和ALP的反应中3-diaminophenazine (DAP) 的光增强.
开发了一个智能手机辅助的视觉检测模型,使用DAP颜色变化的R/B比率.

主要成果:

实现了Cu²⁺的敏感检测,检测极限 (LOD) 为2nM,ALP的LOD为0.03U/mL.
在增样本中证明了Cu²⁺和ALP的可接受恢复率.
确定Na₃VO₄的IC50为ALP抑制的25μM,并建立基于智能手机的视觉检测LODs为Cu^{2+的0.1μM和ALP的0.18U/mL.}

结论:

开发的双模式传感器提供了一个简单,灵活和敏感的平台来检测Cu2+和ALP.
智能手机辅助的视觉检测能力在临床诊断和高通量药物查方面具有重大潜力.

关键词:

在阿尔卑斯山,阿尔卑斯山.在 AMT 中,AMT 是 AMT 的代名词.在Cu2++中.R/B/R/B 是一个字符.比率-光传感器传感器

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