光条形编码和流量计基于光发射器的暗态转换.
Elin Sandberg1, Baris Demirbay1, Abhilash Kulkarni1
1Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Dept. Applied Physics, Albanova University Center, 106 91 Stockholm, Sweden.
The journal of physical chemistry. B
|December 21, 2023
概括
这项研究使用光闪动动力学来区分Cyanine5 (Cy5) 和罗达胺染料. 过渡状态光谱学 (TRAST) 能够在活细胞和微流体样本中进行多重成像,提供了一个新的条形编码策略.
科学领域:
- 生物物理学的生物物理.
- 频谱学是一种光谱学.
- 光成像技术 光成像技术
背景情况:
- 光体中的可逆暗态过渡限制了超敏感光谱和超分辨率成像.
- 然而,这些转换可以用于基于直角光的读数参数.
- 区分具有重叠的辐射光谱的光体对于多重检测至关重要.
研究的目的:
- 分析Cyanine5 (Cy5) 的闪动力学作为一个条形码功能.
- 根据它们独特的闪行为,区分Cy5和罗达胺光体.
- 开发和应用瞬态光谱 (TRAST) 用于多重成像和分析.
主要方法:
- 光相关谱学 (FCS) 用于分析溶液和囊泡中的光混合物.
- 过渡状态 (TRAST) 光谱法通过激发调制来确定光体暗状态动力学.
- 活细胞和微流体系统的广场成像使用TRAST进行空间分辨多重复合.
主要成果:
- FCS表明,Cy5的可逆的跨cis异构化允许与罗达胺区分开来.
- TRAST稳定地确定了暗状态动力学,并使Cy5和Rhodamines在细胞水平和空间解决的歧视成为可能.
- 一个微流体TRAST概念成功地在飞行中区分了光混合物.
结论:
- TRAST提供了一种基于闪动态的强大方法来区分光体,独立于单分子检测或高时间分辨率.
- 这种条形编码概念为各种生物样本的光基复合提供了有价值的策略.
- TRAST广泛适用于静止和移动样本,增强多重光分析.
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