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Updated: Jul 7, 2025

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酶反应辅助可编程的转录开关用于生物活性分子检测

Xiaochen Tang1,2,3, Tianshu Chen1,3, Yonggeng Ma4

  • 1Department of Clinical Laboratory Medicine, Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P. R. China.

Analytical chemistry
|December 21, 2023
PubMed
概括

这项研究引入了新的酶反应辅助转录开关,用于检测过氧化 (H2O2) 等生物活性分子. 这种可编程方法为体外和体内生物应用提供了特异性和抗干扰性.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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