皮布2是一种囊蛋白传感器,参与Saccharomyces cerevisiae中的TORC1激活
Qingzhong Zeng1, Yasuhiro Araki2, Takeshi Noda3
1Graduate School of Frontier Biosciences, Osaka University, Osaka 565-0871, Japan.
Cell reports
|December 21, 2023
概括
拉巴胺复合体1 (TORC1) 的标通过感知氨基酸来调节细胞生长. 这项研究揭示了氨酸通过Pib2通道直接激活TORC1,识别Pib2作为氨酸传感器.
科学领域:
- 细胞生物学 细胞生物学
- 营养物质感应的分子机制
- 酵母作为一个模型生物的酵母.
背景情况:
- 拉巴胺复合体1 (TORC1) 的标对细胞生长至关重要,它集成营养可用性信号.
- 在Saccharomyces cerevisiae中TORC1的激活涉及Gtr和Pib2通路.
- 氨基酸的可用性决定了TORC1通路的利用.
研究的目的:
- 阐明Gtr和Pib2通路在单个氨基酸的TORC1激活中的特定作用.
- 研究囊素影响TORC1信号传递的机制.
- 为了确定氨酸与其传感器蛋白之间的直接相互作用.
主要方法:
- 对TORC1基质Ser3进行酸化分析,以表明通路活性.
- 使用基板Sch9.9评估TORC1活动.
- 在体内和体外的TORC1,Pib2和氨酸之间的相互作用研究.
- 局部定向的突变发生,以确定Pib2.中的关键残留物.
主要成果:
- 不同的氨基酸对TORC1激活的Gtr和Pib2通路具有不同的依赖性.
- 氨酸对Pib2通路具有很强的依赖性.
- 半氨酸在T ((ail) 基因中的W632和F635残留物中直接与Pib2结合,增强TORC1-Pib2相互作用.
- Pib2 作为氨酸的直接传感器.
结论:
- Pib2被确定为TORC1调节的特定的囊蛋白传感器.
- 氨酸通过Pib2介导的信号直接激活TORC1.
- 这项研究揭示了氨基酸感应在营养调节成长中的新机制.
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